ArticleCurrent genetics2006
Consecutive gene deletions in Aspergillus nidulans: application of the Cre/loxP system.
Article in Current genetics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 52 citations in OpenAlex.
- The visualizable and marker-free gene editing platform mediated by CRISPR/Cas9 in Coleophoma empetri.Applied microbiology and biotechnology · 2026Article
- Constructing pyrG marker by CRISPR/Cas facilities the highly-efficient precise genome editing on industrial Aspergillus niger strain.Bioprocess and biosystems engineering · 2025Article
- Robust Cre recombinase activity in the biotrophic smut fungus Ustilago maydis enables efficient conditional null mutants in planta.Genetics · 2022Article
- Upgrading of efficient and scalable CRISPR-Cas-mediated technology for genetic engineering in thermophilic fungusBiotechnology for biofuels · 2019Article
- New Plasmids forFrontiers in microbiology · 2018Article
- HisB as novel selection marker for gene targeting approaches in Aspergillus niger.BMC microbiology · 2017Article
- Light-inducible genetic engineering and control of non-homologous end-joining in industrial eukaryotic microorganisms: LML 3.0 and OFN 1.0.Scientific reports · 2016Article
- A Tet-on and Cre-loxP Based Genetic Engineering System for Convenient Recycling of Selection Markers in Penicillium oxalicum.Frontiers in microbiology · 2016Article
- Simple and efficient recycling of fungal selectable marker genes with the Cre-loxP recombination system via anastomosis.Fungal genetics and biology : FG & B · 2013Article
- Modified Cre-loxP recombination in Aspergillus oryzae by direct introduction of Cre recombinase for marker gene rescue.Applied and environmental microbiology · 2012Article
- Deciphering metabolic traits of the fungal pathogen Aspergillus fumigatus: redundancy vs. essentiality.Frontiers in microbiology · 2012Article
- Transformation system for Hypocrea jecorina (Trichoderma reesei) that favors homologous integration and employs reusable bidirectionally selectable markers.Applied and environmental microbiology · 2011Article
- Validation of a self-excising marker in the human pathogen Aspergillus fumigatus by employing the beta-rec/six site-specific recombination system.Applied and environmental microbiology · 2010Article
- Application of the Saccharomyces cerevisiae FLP/FRT recombination system in filamentous fungi for marker recycling and construction of knockout strains devoid of heterologous genes.Applied and environmental microbiology · 2010Article
- Tools for fungal proteomics: multifunctional neurospora vectors for gene replacement, protein expression and protein purification.Genetics · 2009Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to perform multiple gene deletions is an important tool for conducting functional genomics. We report the development of a sequential gene deletion protocol for the filamentous fungus Aspergillus nidulans using the Cre/loxP recombinase system of bacteriophage P1. A recyclable genetic marker has been constructed by incorporating loxP direct repeats either side of the Neurospora crassa pyr-4 gene (encodes orotidine 5'-monophosphate decarboxylase) which is able to complement the A. nidulans pyrG89 mutation. This construct can be directed to delete specific genomic regions by attaching flanking sequences corresponding to the desired target. The pyr-4 marker can subsequently be eliminated by Cre-catalysed recombination between the loxP sites. The recombinase gene (cre), which has been placed under the control of the A. nidulans xlnA (xylanase A) gene promoter thus providing a means to switch on (xylose induction) or off (glucose repression) recombinase expression, has been integrated into the genome of an A. nidulans mutant strain defective in orotidine 5'-monophosphate decarboxylase activity (pyrG89). We demonstrate the effectiveness of our deletion system by sequentially deleting two genes, yellow (yA) and white (wA), involved in the synthesis of conidial pigment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.