ArticleGenetics2006
New alleles of SIR2 define cell-cycle-specific silencing functions.
Article in Genetics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Global Transcriptomic Analysis Identifies Gene Expression Programs Regulated by α-Factor in Yeast.Journal of microbiology and biotechnology · 2025Article
- Review
- Yeast mismatch repair components are required for stable inheritance of gene silencing.PLoS genetics · 2020Article
- Functional complementation of sir2Δ yeast mutation by the human orthologous gene SIRT1.PloS one · 2013Article
- Yeast Tdh3 (glyceraldehyde 3-phosphate dehydrogenase) is a Sir2-interacting factor that regulates transcriptional silencing and rDNA recombination.PLoS genetics · 2013Article
- H2A.Z (Htz1) controls the cell-cycle-dependent establishment of transcriptional silencing at Saccharomyces cerevisiae telomeres.Genetics · 2011Article
- Proliferating cell nuclear antigen (PCNA) is required for cell cycle-regulated silent chromatin on replicated and nonreplicated genes.The Journal of biological chemistry · 2010Article
- A yeast sir2 mutant temperature sensitive for silencing.Genetics · 2008Article
- HST3/HST4-dependent deacetylation of lysine 56 of histone H3 in silent chromatin.Molecular biology of the cell · 2008Article
- Synthetic lethal screens identify gene silencing processes in yeast and implicate the acetylated amino terminus of Sir3 in recognition of the nucleosome core.Molecular and cellular biology · 2008Article
- Conversion of a replication origin to a silencer through a pathway shared by a Forkhead transcription factor and an S phase cyclin.Molecular biology of the cell · 2008Article
- Bypassing the catalytic activity of SIR2 for SIR protein spreading in Saccharomyces cerevisiae.Molecular biology of the cell · 2006Article
- Asymmetric positioning of nucleosomes and directional establishment of transcriptionally silent chromatin by Saccharomyces cerevisiae silencers.Molecular and cellular biology · 2006Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The establishment of transcriptional silencing in yeast requires cell-cycle progression, but the nature of this requirement is unknown. Sir2 is a protein deacetylase that is required for gene silencing in yeast. We have used temperature-sensitive alleles of the SIR2 gene to assess Sir2's contribution to silencing as a function of the cell cycle. When examined in vivo, these conditional alleles fall into two classes: one class exhibits a loss of silencing when raised to the nonpermissive temperature regardless of cell-cycle position, while the second class exhibits a mitosis-specific silencing defect. Alleles of the first class have a primary defect in protein deacetylase activity, while the alleles of the second class are specifically defective in Sir2-Sir4 interactions at nonpermissive temperatures. Using a SIR2 temperature-sensitive allele, we show that silencing can be established at the HML locus during progression through the G2/M-G1 interval. These results suggest that yeast heterochromatin undergoes structural transitions as a function of the cell cycle and support the existence of a critical assembly step for silent chromatin in mitosis.
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