ArticleGenetics2006
The Saccharomyces cerevisiae rev6-1 mutation, which inhibits both the lesion bypass and the recombination mode of DNA damage tolerance, is an allele of POL30, encoding proliferating cell nuclear antigen.
Article in Genetics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- The inner side of yeast PCNA contributes to genome stability by mediating interactions with Rad18 and the replicative DNA polymerase δ.Scientific reports · 2022Article
- PrimPol-dependent single-stranded gap formation mediates homologous recombination at bulky DNA adducts.Nature communications · 2020Article
- Review
- Identification of New Mutations at the PCNA Subunit Interface that Block Translesion Synthesis.PloS one · 2016Article
- Characterization of proliferating cell nuclear antigen (PCNA) from pathogenic yeast Candida albicans and its functional analyses in S. cerevisiae.BMC microbiology · 2015Article
- Dead-End Elimination with a Polarizable Force Field Repacks PCNA Structures.Biophysical journal · 2015Article
- Structurally distinct ubiquitin- and sumo-modified PCNA: implications for their distinct roles in the DNA damage response.Structure (London, England : 1993) · 2015Article
- Distinct structural alterations in proliferating cell nuclear antigen block DNA mismatch repair.Biochemistry · 2013Article
- PCNA trimer instability inhibits translesion synthesis by DNA polymerase η and by DNA polymerase δ.DNA repair · 2013Article
- PCNA structure and function: insights from structures of PCNA complexes and post-translationally modified PCNA.Sub-cellular biochemistry · 2012Review
- Solution X-ray scattering combined with computational modeling reveals multiple conformations of covalently bound ubiquitin on PCNA.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The non-canonical protein binding site at the monomer-monomer interface of yeast proliferating cell nuclear antigen (PCNA) regulates the Rev1-PCNA interaction and Polζ/Rev1-dependent translesion DNA synthesis.The Journal of biological chemistry · 2011Article
- Crystal structure of SUMO-modified proliferating cell nuclear antigen.Journal of molecular biology · 2011Article
- Processivity factor of DNA polymerase and its expanding role in normal and translesion DNA synthesis.Biochimica et biophysica acta · 2010Review
- SBF transcription factor complex positively regulates UV mutagenesis in Saccharomyces cerevisiae.Biochemical and biophysical research communications · 2009Article
- Separate roles of structured and unstructured regions of Y-family DNA polymerases.Advances in protein chemistry and structural biology · 2009Review
- Structure of a mutant form of proliferating cell nuclear antigen that blocks translesion DNA synthesis.Biochemistry · 2008Article
- Crystallographic study of G178S mutant of human proliferating cell nuclear antigen.Acta crystallographica. Section F, Structural biology and crystallization communications · 2008Article
- Contributions of ubiquitin- and PCNA-binding domains to the activity of Polymerase eta in Saccharomyces cerevisiae.Nucleic acids research · 2007Article
- A novel function of DNA polymerase zeta regulated by PCNA.The EMBO journal · 2006Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The rev6-1 allele was isolated in a screen for mutants deficient for UV-induced reversion of the frameshift mutation his4-38. Preliminary testing showed that the rev6-1 mutant was substantially deficient for UV-induced reversion of arg4-17 and ilv1-92 and markedly UV sensitive. Unlike other REV genes, which encode DNA polymerases and an associated subunit, REV6 has been found to be identical to POL30, which encodes proliferating cell nuclear antigen (PCNA), the subunit of the homotrimeric sliding clamp, in which the rev6-1 mutation produces a G178S substitution. This substitution appears to abolish all DNA damage-tolerance activities normally carried out by the RAD6/RAD18 pathway, including translesion replication by DNA polymerase zeta/Rev1 and DNA polymerase eta, and the error-free, recombination-dependent component of this pathway, but has little effect on the growth rate, suggesting that G178S may prevent ubiquitination of lysine 164 in PCNA. We also find that rev6-1 mutation can be fully complemented by a centromere-containing, low copy-number plasmid carrying POL30, despite the presumed occurrence in the mutant of sliding clamp assemblies that contain between one and three G178S PCNA monomers as well as the fully wild-type species.
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