ArticleMolecular and cellular biology2006
Essential domains for ribonucleoprotein complex formation required for retrotransposition of telomere-specific non-long terminal repeat retrotransposon SART1.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Completion of LINE integration involves an open '4-way' branched DNA intermediate.Nucleic acids research · 2019Article
- Both the Exact Target Site Sequence and a Long Poly(A) Tail Are Required for Precise Insertion of the 18S Ribosomal DNA-Specific Non-Long Terminal Repeat Retrotransposon R7Ag.Molecular and cellular biology · 2016Article
- The spliceosome factor SART1 exerts its anti-HCV action through mRNA splicing.Journal of hepatology · 2015Article
- Integrated mechanism for the generation of the 5' junctions of LINE inserts.Nucleic acids research · 2014Article
- Modular organization and reticulate evolution of the ORF1 of Jockey superfamily transposable elements.Mobile DNA · 2014Article
- Mechanism by which a LINE protein recognizes its 3' tail RNA.Nucleic acids research · 2014Article
- Lost in translation: The biogenesis of non-LTR retrotransposon proteins.Mobile genetic elements · 2013Article
- Self-interaction, nucleic acid binding, and nucleic acid chaperone activities are unexpectedly retained in the unique ORF1p of zebrafish LINE.Molecular and cellular biology · 2012Article
- Genetic evidence that the non-homologous end-joining repair pathway is involved in LINE retrotransposition.PLoS genetics · 2009Article
- Article
- Characterization of the sequence specificity of the R1Bm endonuclease domain by structural and biochemical studies.Nucleic acids research · 2007Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Non-long terminal repeat (LTR) retrotransposons are major components of the higher eukaryotic genome. Most of them have two open reading frames (ORFs): ORF2 encodes mainly the endonuclease and reverse transcriptase domains, but the functional features of ORF1 remain largely unknown. We used telomere-specific non-LTR retrotransposon SART1 in Bombyx mori and clarified essential roles of the ORF1 protein (ORF1p) in ribonucleoprotein (RNP) formation by novel approaches: in vitro reconstitution and in vivo/in vitro retrotransposition assays using the baculovirus expression system. Detailed mutation analyses showed that each of the three CCHC motifs at the ORF1 C terminus are essential for SART1 retrotransposition and are involved in packaging the SART1 mRNA specifically into RNP. We also demonstrated that amino acid residues 555 to 567 and 285 to 567 in the SART1 ORF1p are crucial for the ORF1p-ORF1p and ORF1p-ORF2p interactions, respectively. The loss of these domains abolishes protein-protein interaction, leading to SART1 retrotransposition deficiency. These data suggest that systematic formation of RNP composed of ORF1p, ORF2p, and mRNA is mainly mediated by ORF1p domains and is a common, essential step for many non-LTR retrotransposons encoding the two ORFs.
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