ArticleMolecular and cellular biology2006
Fine-structure analysis of ribosomal protein gene transcription.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 99 citations in OpenAlex.
- Regulation of the Growth-Inhibitory Activity of Fhl1 via Interaction With Ifh1 and Crf1 at the Ribosomal Protein Gene Promoters in Saccharomyces cerevisiae.Genes to cells : devoted to molecular & cellular mechanisms · 2026Article
- Article
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- Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism.Nucleic acids research · 2022Article
- Interaction of TOR and PKA Signaling inBiomolecules · 2022Review
- Yeast Crf1p: An activator in need is an activator indeed.Computational and structural biotechnology journal · 2022Review
- Regulation of ribosomal protein genes: An ordered anarchy.Wiley interdisciplinary reviews. RNA · 2021Review
- Mechanisms coordinating ribosomal protein gene transcription in response to stress.Nucleic acids research · 2020Article
- The TOR pathway modulates cytoophidium formation inThe Journal of biological chemistry · 2019Article
- Global translation inhibition yields condition-dependent de-repression of ribosome biogenesis mRNAs.Nucleic acids research · 2019Article
- Cycloheximide can distort measurements of mRNA levels and translation efficiency.Nucleic acids research · 2019Article
- In Vivo-In Vitro Comparative Toxicology of Cadmium Sulphide Quantum Dots in the Model OrganismNanomaterials (Basel, Switzerland) · 2019Article
- Feedback regulation of ribosome assembly.Current genetics · 2018Review
- Control of RNA polymerase II-transcribed genes by direct binding of TOR kinase.Current genetics · 2018Review
- The ribosome assembly gene network is controlled by the feedback regulation of transcription elongation.Nucleic acids research · 2017Article
- DNA damage regulates direct association of TOR kinase with the RNA polymerase II-transcribedMolecular biology of the cell · 2017Article
- Identification of a transcriptional activation domain in yeast repressor activator protein 1 (Rap1) using an altered DNA-binding specificity variant.The Journal of biological chemistry · 2017Article
- Promoter architecture and transcriptional regulation of Abf1-dependent ribosomal protein genes in Saccharomyces cerevisiae.Nucleic acids research · 2016Article
- Pluralistic and stochastic gene regulation: examples, models and consistent theory.Nucleic acids research · 2016Article
- Molecular mechanisms of ribosomal protein gene coregulation.Genes & development · 2015Article
5 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The ribosomal protein genes of Saccharomyces cerevisiae, responsible for nearly 40% of the polymerase II transcription initiation events, are characterized by the constitutive tight binding of the transcription factor Rap1. Rap1 binds at many places in the yeast genome, including glycolytic enzyme genes, the silent MAT loci, and telomeres, its specificity arising from specific cofactors recruited at the appropriate genes. At the ribosomal protein genes two such cofactors have recently been identified as Fhl1 and Ifh1. We have now characterized the interaction of these factors at a bidirectional ribosomal protein promoter by replacing the Rap1 sites with LexA operator sites. LexA-Gal4(AD) drives active transcription at this modified promoter, although not always at the correct initiation site. Tethering Rap1 to the promoter neither drives transcription nor recruits Fhl1 or Ifh1, showing that Rap1 function requires direct DNA binding. Tethering Fhl1 also fails to activate transcription, even though it does recruit Ifh1, suggesting that Fhl1 does more than simply provide a platform for Ifh1. Tethering Ifh1 to the promoter leads to low-level transcription, at the correct initiation sites. Remarkably, activation by tethered LexA-Gal4(AD) is strongly reduced when TOR kinase is inhibited by rapamycin. Thus, TOR can act independently of Fhl1/Ifh1 at ribosomal protein promoters. We also show that, in our strain background, the response of ribosomal protein promoters to TOR inhibition is independent of the Ifh1-related protein Crf1, indicating that the role of this corepressor is strain specific. Fine-structure chromatin mapping of several ribosomal protein promoters revealed that histones are essentially absent from the Rap1 sites, while Fhl1 and Ifh1 are coincident with each other but distinct from Rap1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.