ArticleBiophysical journal2006
Channel activity of OmpF monitored in nano-BLMs.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 98 citations in OpenAlex.
- Aquaporin embedded suspended lipid bilayer on anodized alumina nanoporous substrates for studying stability and functionality: towards the development of a miniaturized water purifier.Nanoscale advances · 2026Article
- Dual function of OmpM as outer membrane tether and nutrient uptake channel in diderm Firmicutes.Nature communications · 2023Article
- The Whole Is Bigger than the Sum of Its Parts: Drug Transport in the Context of Two Membranes with Active Efflux.Chemical reviews · 2021Article
- Nanodomain Formation in Planar Supported Lipid Bilayers Composed of Fluid and Polymerized Dienoyl Lipids.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Structural basis for chitin acquisition by marine Vibrio species.Nature communications · 2018Article
- Reconstitution of the membrane protein OmpF into biomimetic block copolymer-phospholipid hybrid membranes.Beilstein journal of nanotechnology · 2016Article
- Applications of biological pores in nanomedicine, sensing, and nanoelectronics.Current opinion in biotechnology · 2010Review
- Fractional polymerization of a suspended planar bilayer creates a fluid, highly stable membrane for ion channel recordings.Journal of the American Chemical Society · 2010Article
- The M34A mutant of Connexin26 reveals active conductance states in pore-suspending membranes.Journal of structural biology · 2009Article
- Enhanced long-term stability for single ion channel recordings using suspended poly(lipid) bilayers.Journal of the American Chemical Society · 2009Article
- Modulation of the conductance of a 2,2'-bipyridine-functionalized peptidic ion channel by Ni2+.European biophysics journal : EBJ · 2008Article
- Controlled delivery of proteins into bilayer lipid membranes on chip.Lab on a chip · 2007Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Free-standing lipid bilayer membranes can be formed on small apertures (60 nm diameter) on highly ordered porous alumina substrates. The formation process of the membranes on a 1,2-dipalmitoyl-sn-glycero-3-phosphothioethanol submonolayer was followed by impedance spectroscopy. After lipid bilayers had thinned, the reconstitution and ionic conducting properties of the outer membrane protein OmpF of E. coli were monitored using single-channel recordings. The characteristic conductance states of the three monomers, fast kinetics, and subconductance states were observed. Blockade of the ion flow as a result of interaction of the antibiotic ampicillin with the protein was verified, indicating the full functionality of the protein channel in nanometer-scale bilayer membranes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.