ArticleCurrent biology : CB2006
Kinetochores use a novel mechanism for coordinating the dynamics of individual microtubules.
Article in Current biology : CB, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 74 citations in OpenAlex.
- Chromosome segregation synchrony in S. pombe is noise limited and arises without positive feedback.The Journal of cell biology · 2026Article
- Chromosome segregation synchrony inbioRxiv : the preprint server for biology · 2026Article
- On the curvature and relaxation of microtubule plus-end tips.Biophysical journal · 2026Article
- Mammalian metaphase kinetochores are elastic and require condensin for robust structure and function.bioRxiv : the preprint server for biology · 2025Article
- Article
- Mechanical coupling coordinates microtubule growth.bioRxiv : the preprint server for biology · 2023Article
- MCRS1 modulates the heterogeneity of microtubule minus-end morphologies in mitotic spindles.Molecular biology of the cell · 2023Article
- Regulation of microtubule dynamics, mechanics and function through the growing tip.Nature reviews. Molecular cell biology · 2021Review
- Mechanisms of microtubule dynamics and force generation examined with computational modeling and electron cryotomography.Nature communications · 2020Article
- Three-dimensional organization of the cytoskeleton: A cryo-electron tomography perspective.Protein science : a publication of the Protein Society · 2020Review
- Microtubules grow by the addition of bent guanosine triphosphate tubulin to the tips of curved protofilaments.The Journal of cell biology · 2018Article
- Mitotic spindle: kinetochore fibers hold on tight to interpolar bundles.European biophysics journal : EBJ · 2018Review
- Mechanisms of Chromosome Congression during Mitosis.Biology · 2017Review
- Review
- Chromosome biorientation produces hundreds of piconewtons at a metazoan kinetochore.Nature communications · 2016Article
- Aurora-A regulates MCRS1 function during mitosis.Cell cycle (Georgetown, Tex.) · 2016Article
- Article
- Inferring the Forces Controlling Metaphase Kinetochore Oscillations by Reverse Engineering System Dynamics.PLoS computational biology · 2015Article
- Article
- Minimal model for collective kinetochore-microtubule dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
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6 authors at 3 institutions in 2 countries.
Funding
Abstract
Chromosome alignment during mitosis is frequently accompanied by a dynamic switching between elongation and shortening of kinetochore fibers (K-fibers) that connect kinetochores and spindle poles . In higher eukaryotes, mature K-fibers consist of 10-30 kinetochore microtubules (kMTs) whose plus ends are embedded in the kinetochore . A critical and long-standing question is how the dynamics of individual kMTs within the K-fiber are coordinated . We have addressed this question by using electron tomography to determine the polymerization/depolymerization status of individual kMTs in the K-fibers of PtK1 and Drosophila S2 cells. Surprisingly, we find that the plus ends of two-thirds of kMTs are in a depolymerizing state, even when the K-fiber exhibits net tubulin incorporation at the plus end . Furthermore, almost all individual K-fibers examined had a mixture of kMTs in the polymerizing and depolymerizing states. Therefore, although K-fibers elongate and shrink as a unit, the dynamics of individual kMTs within a K-fiber are not coordinated at any given moment. Our results suggest a novel control mechanism through which attachment to the kinetochore outer plate prevents shrinkage of kMTs. We discuss the ramifications of this new model on the regulation of chromosome movement and the stability of K-fibers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.