ArticleJournal of clinical immunology2006
Cloning, recombinant expression and activity studies of a major allergen "enolase" from the fungus Curvularia lunata.
Article in Journal of clinical immunology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 25 citations in OpenAlex.
- Problems Encountered Using Fungal Extracts as Test Solutions for Fungal Allergy Diagnosis.Journal of fungi (Basel, Switzerland) · 2023Review
- Sensitization against Fungi in Patients with Airway Allergies over 20 Years in Germany.International archives of allergy and immunology · 2021Review
- Review
- Proteomic Analysis and Identification of Possible Allergenic Proteins in Mature Pollen of Populus tomentosa.International journal of molecular sciences · 2018Article
- Comparative Proteomic Analysis of Mature Pollen in Triploid and Diploid Populus deltoides.International journal of molecular sciences · 2016Article
- Structural aspects of fungal allergens.Seminars in immunopathology · 2015Review
- Production of a Chaetomium globosum enolase monoclonal antibody.Monoclonal antibodies in immunodiagnosis and immunotherapy · 2014Article
- Identification of B cell epitopes of alcohol dehydrogenase allergen of Curvularia lunata.PloS one · 2011Article
- Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana.Clinical and molecular allergy : CMA · 2006Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recombinant allergens are required to study allergy at the molecular level and are helpful tools for the improvement of diagnosis and therapy. In the present study, enolase was expressed from Curvularia lunata and analyzed for its immunological reactivity as an allergen. cDNA library was synthesized in lambda zap vector and screened with sera obtained from C. lunata allergic patients. A cDNA clone with an ORF of 1.3 kb showed homology to enolases from different fungal sources. It was expressed in E. coli, purified from inclusion bodies yielding 0.5 mg/L and showed enzyme activity of 48 units/mg. It resolved as 48-kDa band on SDS-PAGE and was recognized by all the individual Curvularia positive patient sera in immunoblot and ELISA. r Cur l 2 stimulated patients' PBMCs and supernatant of these cells showed elevated levels of Th 2 cytokines. Ten B cell epitopes were predicted using computational software and one showed 90% homology to an important IgE epitope of Cla h 6. The various parameters predicted by computational approach can be validated later as a future study to draw conclusive evidence about putative antigenic epitopes. This can further help in generating knowledge about residues important for IgE binding and developing therapeutic modalities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.