ArticleTransgenic research2006
Glycosylated F4 (K88) fimbrial adhesin FaeG expressed in barley endosperm induces ETEC-neutralizing antibodies in mice.
Article in Transgenic research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 50 citations in OpenAlex.
- Human β-Defensin 118 Attenuates Escherichia coli K88-Induced Inflammation and Intestinal Injury in Mice.Probiotics and antimicrobial proteins · 2021Article
- Recombinant Plant Engineering for Immunotherapeutic Production.Current molecular biology reports · 2017Review
- Animal Enterotoxigenic Escherichia coli.EcoSal Plus · 2016Review
- Farming of Plant-Based Veterinary Vaccines and Their Applications for Disease Prevention in Animals.Advances in virology · 2015Review
- Plant-based solutions for veterinary immunotherapeutics and prophylactics.Veterinary research · 2014Review
- Article
- Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco.PloS one · 2012Article
- Expression of anti-K99 scFv in transgenic rice tissues and its functional characterization.Transgenic research · 2009Article
- Oral immunogenicity and protective efficacy in mice of transgenic rice plants producing a vaccine candidate antigen (As16) of Ascaris suum fused with cholera toxin B subunit.Transgenic research · 2009Article
- Viral vectors for production of recombinant proteins in plants.Journal of cellular physiology · 2008Review
- Transgenic plants for animal health: plant-made vaccine antigens for animal infectious disease control.Phytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2008Article
- Production of vaccines and therapeutic antibodies for veterinary applications in transgenic plants: an overview.Transgenic research · 2007Review
- Optimized FaeG expression and a thermolabile enterotoxin DNA adjuvant enhance priming of an intestinal immune response by an FaeG DNA vaccine in pigs.Clinical and vaccine immunology : CVI · 2007Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The F4-positive enterotoxigenic Escherichia coli (ETEC) strains are a frequent cause of porcine post-weaning diarrhea. Orally administered F4 fimbriae or FaeG, the major subunit and adhesin of F4, induce a protective mucosal immune response in F4 receptor-positive piglets. Feed plants carrying immunogenic subunit proteins can offer great advantages for oral vaccination of domestic animals. Here, we describe high-level endosperm-specific production (1% of total soluble proteins) of FaeG in the crop plant barley. The endoplasmic reticulum-targeted recombinant endospermic FaeG (erFaeG) was shown to be heterogeneously glycosylated. The erFaeG showed resistance at digestive conditions simulating piglet gastric fluid. Glycosylation did not abolish the immunogenic character of the FaeG protein, since erFaeG was able to induce F4 fimbria-specific antibodies in mice. Biological activity of these anti-F4 antibodies was demonstrated in vitro by blocking the attachment of the F4+ ETEC to the F4 receptors present on porcine intestinal enterocytes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.