ArticleThe EMBO journal2006
Rapid accessibility of nucleosomal DNA in yeast on a second time scale.
Article in The EMBO journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Structural basis of PARP2 recognition of nucleosomal DNA breaks and inhibitor trapping.Nucleic acids research · 2026Article
- The yeast genome is globally accessible in living cells.Nature structural & molecular biology · 2025Article
- Genome-wide mapping of genomic DNA damage: methods and implications.Cellular and molecular life sciences : CMLS · 2021Review
- Molecular dynamics simulations reveal how H3K56 acetylation impacts nucleosome structure to promote DNA exposure for lesion sensing.DNA repair · 2021Article
- Dynamics of Chromatin and Transcription during Transient Depletion of the RSC Chromatin Remodeling Complex.Cell reports · 2019Article
- Nucleosome DNA unwrapping does not affect prototype foamy virus integration efficiency or site selection.PloS one · 2019Article
- Intracellular nucleosomes constrain a DNA linking number difference of -1.26 that reconciles the Lk paradox.Nature communications · 2018Article
- H4 Tails Potentially Produce the Diversity in the Orientation of Two Nucleosomes.Biophysical journal · 2017Article
- Dynamic chromatin technologies: from individual molecules to epigenomic regulation in cells.Nature reviews. Genetics · 2017Review
- UV-Induced DNA Damage and Mutagenesis in Chromatin.Photochemistry and photobiology · 2017Review
- Repair of Alkylation Damage in Eukaryotic Chromatin Depends on Searching Ability of Alkyladenine DNA Glycosylase.ACS chemical biology · 2015Article
- Accessing DNA damage in chromatin: Preparing the chromatin landscape for base excision repair.DNA repair · 2015Review
- Chromatin imaging with time-lapse atomic force microscopy.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Nanoscale Nucleosome Dynamics Assessed with Time-lapse AFM.Biophysical reviews · 2014Article
- Histone H3 lysine 14 (H3K14) acetylation facilitates DNA repair in a positioned nucleosome by stabilizing the binding of the chromatin Remodeler RSC (Remodels Structure of Chromatin).The Journal of biological chemistry · 2014Article
- The yeast PHO5 promoter: from single locus to systems biology of a paradigm for gene regulation through chromatin.Nucleic acids research · 2014Review
- Regulation of the nucleosome unwrapping rate controls DNA accessibility.Nucleic acids research · 2012Article
- Unwrapping of nucleosomal DNA ends: a multiscale molecular dynamics study.Biophysical journal · 2012Article
- Participation of chromatin-remodeling proteins in the repair of ultraviolet-B-damaged DNA.Plant physiology · 2012Article
- Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Packaging DNA in nucleosomes and higher-order chromatin structures restricts its accessibility and constitutes a barrier for all DNA transactions including gene regulation and DNA repair. How and how fast proteins find access to DNA buried in chromatin of living cells is poorly understood. To address this question in a real time in vivo approach, we investigated DNA repair by photolyase in yeast. We show that overexpressed photolyase, a light-dependent DNA-repair enzyme, recognizes and repairs UV-damaged DNA within seconds. Rapid repair was observed in various nucleosomal regions of the genome including inactive and active genes and repressed promoters. About 50% of cyclobutane pyrimidine dimers were removed in 5 s, >80% in 90 s. Heterochromatin was repaired within minutes, centromeres were not repaired. Consistent with fast conformational transitions of nucleosomes observed in vitro, this rapid repair strongly suggests that spontaneous unwrapping of nucleosomes rather than histone dissociation or chromatin remodeling provides DNA access. The data impact our view on the repressive and dynamic nature of chromatin and illustrate how proteins like photolyase can access DNA in structurally and functionally diverse chromatin regions.
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