ArticleProceedings of the National Academy of Sciences of the United States of America2006
Regulation of nitrogenase by 2-oxoglutarate-reversible, direct binding of a PII-like nitrogen sensor protein to dinitrogenase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 76 citations in OpenAlex.
- Article
- Molecular basis of NNature communications · 2026Article
- Function, Structure, and Regulation of Nitrogen Fixation-like Metalloproteins for Nitrogen, Energy, Carbon, and Sulfur Metabolism.Chemical reviews · 2026Review
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- ModE Regulates Alternative Nitrogenase Expression in the Methanogen Methanosarcina acetivorans.Molecular microbiology · 2025Article
- Article
- An improved CRISPR and CRISPR interference (CRISPRi) toolkit for engineering the model methanogenic archaeon Methanococcus maripaludis.Microbial cell factories · 2024Article
- Model Organisms To Study Methanogenesis, a Uniquely Archaeal Metabolism.Journal of bacteriology · 2023Review
- Random transposon mutagenesis identifies genes essential for transformation in Methanococcus maripaludis.Molecular genetics and genomics : MGG · 2023Article
- Article
- CRISPR-Cas9 Toolkit for Genome Editing in an Autotrophic COMicrobiology spectrum · 2022Article
- The Fluorescence-Activating and Absorption-Shifting Tag (FAST) Enables Live-Cell Fluorescence Imaging of Methanococcus maripaludis.Journal of bacteriology · 2022Article
- Efficient CRISPR/Cas12a-Based Genome-Editing Toolbox for Metabolic Engineering inACS synthetic biology · 2022Article
- A Genetic Study ofMicrobiology spectrum · 2022Article
- The Oligosaccharyltransferase AglB Supports Surface-Associated Growth and Iron Oxidation in Methanococcus maripaludis.Applied and environmental microbiology · 2021Article
- Type IV-Like Pili Facilitate Transformation in Naturally Competent Archaea.Journal of bacteriology · 2020Article
- Analysis of the Complete Genome of the Alkaliphilic and Phototrophic FirmicuteMicroorganisms · 2020Article
- Small RNAs Involved in Regulation of Nitrogen Metabolism.Microbiology spectrum · 2018Review
- Methanogens Are Major Contributors to Nitrogen Fixation in Soils of the Florida Everglades.Applied and environmental microbiology · 2018Article
- sRNARNA biology · 2017Article
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2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Posttranslational regulation of nitrogenase, or switch-off, in the methanogenic archaeon Methanococcus maripaludis requires both nifI(1) and nifI(2), which encode members of the PII family of nitrogen-regulatory proteins. Previous work demonstrated that nitrogenase activity in cell extracts was inhibited in the presence of NifI(1) and NifI(2), and that 2-oxoglutarate (2OG), a potential signal of nitrogen limitation, relieved this inhibition. To further explore the role of the NifI proteins in switch-off, we found proteins that interact with NifI(1) and NifI(2) and determined whether 2OG affected these interactions. Anaerobic purification of His-tagged NifI(2) resulted in copurification of NifI(1) and the dinitrogenase subunits NifD and NifK, and 2OG or a deletion mutation affecting the T-loop of NifI(2) prevented copurification of dinitrogenase but did not affect copurification of NifI(1). Similar results were obtained with His-tagged NifI(1). Gel-filtration chromatography demonstrated an interaction between purified NifI(1,2) and dinitrogenase that was inhibited by 2OG. The NifI proteins themselves formed a complex of approximately 85 kDa, which appeared to further oligomerize in the presence of 2OG. NifI(1,2) inhibited activity of purified nitrogenase when present in a 1:1 molar ratio to dinitrogenase, and 2OG fully relieved this inhibition. These results suggest a model for switch-off of nitrogenase activity, where direct interaction of a NifI(1,2) complex with dinitrogenase causes inhibition, which is relieved by 2OG. The presence of nifI(1) and nifI(2) in the nif operons of all nitrogen-fixing Archaea and some anaerobic Bacteria suggests that this mode of nitrogenase regulation may operate in a wide variety of diazotrophs.
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