ArticleBiochimica et biophysica acta
Topography and chemical reactivity of the active-inactive transition-sensitive SH-group in the mitochondrial NADH:ubiquinone oxidoreductase (Complex I).
Article in Biochimica et biophysica acta. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 37 citations in OpenAlex.
- Mitochondrial Dysfunctions: A Thread Sewing Together Alzheimer's Disease, Diabetes, and Obesity.Oxidative medicine and cellular longevity · 2019Review
- Glutamate contributes to alcohol hepatotoxicity by enhancing oxidative stress in mitochondria.Journal of bioenergetics and biomembranes · 2017Article
- Activation of respiratory Complex I fromHeliyon · 2017Article
- Selective inhibition of deactivated mitochondrial complex I by biguanides.Biochemistry · 2015Article
- Chemical modifications of respiratory complex I for structural and functional studies.Journal of bioenergetics and biomembranes · 2014Review
- Characterisation of the active/de-active transition of mitochondrial complex I.Biochimica et biophysica acta · 2014Review
- ND3, ND1 and 39kDa subunits are more exposed in the de-active form of bovine mitochondrial complex I.Biochimica et biophysica acta · 2014Article
- Molecular mechanism and physiological role of active-deactive transition of mitochondrial complex I.Biochemical Society transactions · 2013Article
- Lack of oxygen deactivates mitochondrial complex I: implications for ischemic injury?The Journal of biological chemistry · 2009Article
- Architecture of complex I and its implications for electron transfer and proton pumping.Biochimica et biophysica acta · 2009Review
- Identification of the mitochondrial ND3 subunit as a structural component involved in the active/deactive enzyme transition of respiratory complex I.The Journal of biological chemistry · 2008Article
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3 authors at 2 institutions in 2 countries.
Funding
Abstract
The spatial arrangement and chemical reactivity of the activation-dependent thiol in the mitochondrial Complex I was studied using the membrane penetrating N-ethylmaleimide (NEM) and non-penetrating anionic 5,5'-dithiobis-(2-nitrobenzoate) (DTNB) as the specific inhibitors of the enzyme in mitochondria and inside-out submitochondrial particles (SMP). Both NEM and DTNB rapidly inhibited the de-activated Complex I in SMP. In mitochondria NEM caused rapid inhibition of Complex I, whereas the enzyme activity was insensitive to DTNB. In the presence of the channel-forming antibiotic alamethicin, mitochondrial Complex I became sensitive to DTNB. Neither active nor de-activated Complex I in SMP was inhibited by oxidized glutathione (10 mM, pH 8.0, 75 min). The data suggest that the active/de-active transition sulfhydryl group of Complex I which is sensitive to inhibition by NEM is located at the inner membrane-matrix interface. These data include the sidedness dependency of inhibition, effect of pH, ionic strength, and membrane bilayer modification on enzyme reactivity towards DTNB and its neutral analogue.
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