ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2006
Cloning and molecular characterization of three novel LMW-i glutenin subunit genes from cultivated einkorn (Triticum monococcum L.).
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 93 citations in OpenAlex.
- Recent duplications and rare structural variations revealed by comparative sequence analysis of low molecular weight glutenin subunits (LMW-GS) genes re-identified using LMWgsFinder in 26 genomes of the grass family.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Effects of LMW-GS Allelic Variations at theFoods (Basel, Switzerland) · 2025Article
- Polymeric Gluten Proteins as Climate-Resilient Markers of Quality: Can LC-MS/MS Provide Valuable Information about Spring Wheat Grown in Diverse Climates?Journal of agricultural and food chemistry · 2025Article
- Characterization of novel LMW-i genes with nine cysteine residues from Chinese wheat landraces (3 Biotech · 2021Article
- Development of an Optimized MALDI-TOF-MS Method for High-Throughput Identification of High-Molecular-Weight Glutenin Subunits in Wheat.Molecules (Basel, Switzerland) · 2020Article
- Molecular characterization and expression of α-gliadin genes from wheat cultivar Dacke in Bg 250 rice variety.GM crops & food · 2019Article
- Cloning and characterization of a novel low-molecular-weight glutenin subunit gene with an unusual molecular structure ofJournal of genetics · 2018Article
- Improved Method for Reliable HMW-GS Identification by RP-HPLC and SDS-PAGE in Common Wheat Cultivars.Molecules (Basel, Switzerland) · 2017Article
- Low molecular weight glutenin subunit gene Glu-B3h confers superior dough strength and breadmaking quality in wheat (Triticum aestivum L.).Scientific reports · 2016Article
- Comprehensive identification of LMW-GS genes and their protein products in a common wheat variety.Functional & integrative genomics · 2016Article
- Molecular characterization of novel LMW-i glutenin subunit genes from Triticum urartu Thum. ex Gandil.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2015Article
- Novel LMW glutenin subunit genes from wild emmer wheat (Triticum turgidum ssp. dicoccoides) in relation to Glu-3 evolution.Development genes and evolution · 2015Article
- Deletion of the low-molecular-weight glutenin subunit allele Glu-A3a of wheat (Triticum aestivum L.) significantly reduces dough strength and breadmaking quality.BMC plant biology · 2014Article
- The α-gliadin genes from Brachypodium distachyon L. provide evidence for a significant gap in the current genome assembly.Functional & integrative genomics · 2014Article
- Cloning and characterization of low-molecular-weight glutenin subunit alleles from Chinese wheat landraces (Triticum aestivum L.).TheScientificWorldJournal · 2014Article
- Cloning, expression, and evolutionary analysis of α-gliadin genes from Triticum and Aegilops genomes.Journal of applied genetics · 2013Article
- Novel insights into the composition, variation, organization, and expression of the low-molecular-weight glutenin subunit gene family in common wheat.Journal of experimental botany · 2013Article
- Effect of Glu-B3 allelic variation on sodium dodecyl sulfate sedimentation volume in common wheat (Triticum aestivum L.).TheScientificWorldJournal · 2013Article
- Molecular characterization of LMW-GS genes in Brachypodium distachyon L. reveals highly conserved Glu-3 loci in Triticum and related species.BMC plant biology · 2012Article
- Identification and molecular characterisation of HMW glutenin subunit 1By16* in wild emmer.Journal of applied genetics · 2012Article
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three novel low molecular weight (LMW) glutenin subunits from cultivated einkorn (Triticum monococcum L., A(m)A(m), 2n = 2x = 14) were characterized by SDS-PAGE and molecular weights determined by MALDI-TOF-MS. Their coding genes were amplified and cloned with designed AS-PCR primers, revealing three complete gene sequences. All comprised upstream, open reading frame (ORF), downstream and no introns were present. The deduced amino acid sequences showed that all three genes, named as LMW-M1, LMW-M3 and LMW-M5, respectively, belonged to the LMW-i type subunits with the predicted molecular weight between 38.5206 and 38.7028 kDa. They showed high similarity with other LMW-i type genes from hexaploid bread wheats, but also displayed unique features. Particularly, LMW-M5 subunit contained an extra cysteine residue in the C-terminus except for eight conserved cysteines, which resulted from a single-nucleotide polymorphism (SNP) of the T-C transition, namely arginine --> cysteine substitution at position 242 from the N-terminal end. This is the first report that the LMW-i subunit contained nine cysteines residues that could result in a more highly cross-linked and more elastic glutenin suggesting that LMW-M5 gene may associates with good quality properties. In addition, a total of 25 SNPs and one insertions/deletions (InDels) were detected among three LMW-i genes, which could result in significant functional changes in polymer formation of gluten. It is anticipated that these SNPs could be used as reliable genetic markers during wheat quality improvement. The phylogenetic analysis indicated that LMW-i type genes apparently differed from LMW-m and LMW-s type genes and diverged early from the primitive LMW-GS gene family, at about 12.92 million years ago (MYA) while the differentiation of A(m) and A genomes was estimated at 3.98 MYA.
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