ArticleJournal of virology2006
Mycovirus cryphonectria hypovirus 1 elements cofractionate with trans-Golgi network membranes of the fungal host Cryphonectria parasitica.
Article in Journal of virology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 58 citations in OpenAlex.
- Article
- Hypovirus-Induced Phosphorylation of CpIre1 Modulates Unfolded Protein Response and Virulence in Cryphonectria parasitica.Molecular plant pathology · 2026Article
- A Viral RNA Silencing Suppressor Modulates Reactive Oxygen Species Levels to Induce the Autophagic Degradation of Dicer-Like and Argonaute-Like Proteins.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Article
- A novel partitivirus conferring hypovirulence by affecting vesicle transport in the fungusmBio · 2024Article
- Article
- ICTV Virus Taxonomy Profile:The Journal of general virology · 2023Article
- Hypovirus infection induces proliferation and perturbs functions of mitochondria in the chestnut blight fungus.Frontiers in microbiology · 2023Article
- A Transfectable Fusagravirus from a Japanese Strain ofViruses · 2022Article
- Deoxynivalenol Biosynthesis inToxins · 2022Article
- A Novel Heptasegmented Positive-Sense Single-Stranded RNA Virus from the Phytopathogenic FungusJournal of virology · 2022Article
- Molecular characteristics of a novel hypovirus from Trichoderma harzianum.Archives of virology · 2022Article
- The virome from a collection of endomycorrhizal fungi reveals new viral taxa with unprecedented genome organization.Virus evolution · 2020Article
- Article
- Facilitative and synergistic interactions between fungal and plant viruses.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Molecular Characterization of a Novel Polymycovirus FromFrontiers in microbiology · 2020Article
- Virome Identification and Characterization ofFrontiers in microbiology · 2020Article
- Symptomatic plant viroid infections in phytopathogenic fungi.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- CpATG8, a Homolog of Yeast Autophagy Protein ATG8, Is Required for Pathogenesis and Hypovirus Accumulation in the Chest Blight Fungus.Frontiers in cellular and infection microbiology · 2019Article
- Hiding in plain sight: New virus genomes discovered via a systematic analysis of fungal public transcriptomes.PloS one · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mycovirus cryphonectria hypovirus 1 (CHV1) causes proliferation of vesicles in its host, Cryphonectria parasitica, the causal agent of chestnut blight. These vesicles have previously been shown to contain both CHV1 genomic double-stranded RNA (dsRNA) and RNA polymerase activity. To determine the cellular origins of these virus-induced membrane structures, we compared the fractionation of several cellular and viral markers. Results showed that viral dsRNA, helicase, polymerase, and protease p29 copurify with C. parasitica trans-Golgi network (TGN) markers, suggesting that the virus utilizes the fungal TGN for replication. We also show that the CHV1 protease p29 associates with vesicle membranes and is resistant to treatments that would release peripheral membrane proteins. Thus, p29 behaves as an integral membrane protein of the vesicular fraction derived from the fungal TGN. Protease p29 was also found to be fully susceptible to proteolytic digestion in the absence of detergent and, thus, is wholly or predominantly on the cytoplasmic face of the vesicles. Fractionation analysis of p29 deletion variants showed that sequences in the C terminal of p29 mediate membrane association. In particular, the C-terminal portion of the protein (Met-135-Gly-248) is sufficient for membrane association and is enough to direct p29 to the TGN vesicles in the absence of other viral elements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.