ArticleRNA (New York, N.Y.)2006
The conserved AAUAAA hexamer of the poly(A) signal can act alone to trigger a stable decrease in RNA polymerase II transcription velocity.
Article in RNA (New York, N.Y.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed.
- Transcription-dependent polyadenylation signal activity contributes to transcriptomic regulation during Forskolin-induced BeWo cell syncytialization.Frontiers in cell and developmental biology · 2026Article
- Article
- Emerging Roles of RNA 3'-end Cleavage and Polyadenylation in Pathogenesis, Diagnosis and Therapy of Human Disorders.Biomolecules · 2020Review
- Control of RNA Pol II Speed by PNUTS-PP1 and Spt5 Dephosphorylation Facilitates Termination by a "Sitting Duck Torpedo" Mechanism.Molecular cell · 2019Article
- Transcription Dynamics in Living Cells.Annual review of biophysics · 2016Review
- The Nrd1-like protein Seb1 coordinates cotranscriptional 3' end processing and polyadenylation site selection.Genes & development · 2016Article
- Temporal Dissection of Rate Limiting Transcriptional Events Using Pol II ChIP and RNA Analysis of Adrenergic Stress Gene Activation.PloS one · 2015Article
- Fail-safe transcription termination: Because one is never enough.RNA biology · 2015Review
- Genome-wide mapping of yeast RNA polymerase II termination.PLoS genetics · 2014Article
- The Reb1-homologue Ydr026c/Nsi1 is required for efficient RNA polymerase I termination in yeast.The EMBO journal · 2012Article
- Prolonged α-amanitin treatment of cells for studying mutated polymerases causes degradation of DSIF160 and other proteins.RNA (New York, N.Y.) · 2012Article
- Article
- RNA polymerase II pausing downstream of core histone genes is different from genes producing polyadenylated transcripts.PloS one · 2012Article
- Poly(A) signals located near the 5' end of genes are silenced by a general mechanism that prevents premature 3'-end processing.Molecular and cellular biology · 2011Article
- Zebrafish cx30.3: identification and characterization of a gap junction gene highly expressed in the skin.Developmental dynamics : an official publication of the American Association of Anatomists · 2010Article
- Functional cis-regulatory genomics for systems biology.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Poly(A) signal-dependent degradation of unprocessed nascent transcripts accompanies poly(A) signal-dependent transcriptional pausing in vitro.RNA (New York, N.Y.) · 2010Article
- Transcription termination by nuclear RNA polymerases.Genes & development · 2009Article
- Polyadenylation releases mRNA from RNA polymerase II in a process that is licensed by splicing.RNA (New York, N.Y.) · 2009Article
- A genomic analysis of RNA polymerase II modification and chromatin architecture related to 3' end RNA polyadenylation.Genome research · 2008Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
In vivo the poly(A) signal not only directs 3'-end processing but also controls the rate and extent of transcription. Thus, upon crossing the poly(A) signal RNA polymerase II first pauses and then terminates. We show that the G/U-rich region of the poly(A) signal, although required for termination in vivo, is not required for poly(A)-dependent pausing either in vivo or in vitro. Consistent with this, neither CstF, which recognizes the G/U-rich element, nor the polymerase CTD, which binds CstF, is required for pausing. The only part of the poly(A) signal required to direct the polymerase to pause is the AAUAAA hexamer. The effect of the hexamer on the polymerase is long lasting--in many situations polymerases over 1 kb downstream of the hexamer continue to exhibit delayed progress down the template in vivo. The hexamer is the first part of the poly(A) signal to emerge from the polymerase and may play a role independent of the rest of the poly(A) signal in paving the way for subsequent events such as 3'-end processing and termination of transcription.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.