ArticleMolecular biology of the cell2006
MARK2/EMK1/Par-1Balpha phosphorylation of Rab11-family interacting protein 2 is necessary for the timely establishment of polarity in Madin-Darby canine kidney cells.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 59 citations in OpenAlex.
- Rab11FIP1-deficient mice develop spontaneous inflammation and show increased susceptibility to colon damage.American journal of physiology. Gastrointestinal and liver physiology · 2022Article
- PKA-independent vasopressin signaling in renal collecting duct.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Large epigenome-wide association study of childhood ADHD identifies peripheral DNA methylation associated with disease and polygenic risk burden.Translational psychiatry · 2020Article
- Article
- Inhibition of the miR-192/215-Rab11-FIP2 axis suppresses human gastric cancer progression.Cell death & disease · 2018Article
- Overexpression of Rab11-FIP2 in colorectal cancer cells promotes tumor migration and angiogenesis through increasing secretion of PAI-1.Cancer cell international · 2018Article
- The role of microtubules in the regulation of epithelial junctions.Tissue barriers · 2018Review
- MARK3-mediated phosphorylation of ARHGEF2 couples microtubules to the actin cytoskeleton to establish cell polarity.Science signaling · 2017Article
- Interaction of phosphorylated Rab11-FIP2 with Eps15 regulates apical junction composition.Molecular biology of the cell · 2017Article
- Proteomics Screen Identifies Class I Rab11 Family Interacting Proteins as Key Regulators of Cytokinesis.Molecular and cellular biology · 2017Article
- Rab11-FIP1 phosphorylation by MARK2 regulates polarity in MDCK cells.Cellular logistics · 2017Article
- Article
- Rab-mediated vesicle trafficking in cancer.Journal of biomedical science · 2016Review
- Regulation of podocalyxin trafficking by Rab small GTPases in epithelial cells.Small GTPases · 2016Article
- Recycling Endosomes and Viral Infection.Viruses · 2016Review
- Rab11-FIP1A regulates early trafficking into the recycling endosomes.Experimental cell research · 2016Article
- Identification of AMPK Phosphorylation Sites Reveals a Network of Proteins Involved in Cell Invasion and Facilitates Large-Scale Substrate Prediction.Cell metabolism · 2015Article
- Rab11 regulates E-cadherin expression and induces cell transformation in colorectal carcinoma.BMC cancer · 2014Article
- Live imaging of GLUT2 glucose-dependent trafficking and its inhibition in polarized epithelial cysts.Open biology · 2014Article
- Rab11-FIP2 interaction with MYO5B regulates movement of Rab11a-containing recycling vesicles.Traffic (Copenhagen, Denmark) · 2014Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Rab11a, myosin Vb, and the Rab11-family interacting protein 2 (FIP2) regulate plasma membrane recycling in epithelial cells. This study sought to characterize more fully Rab11-FIP2 function by identifying kinase activities modifying Rab11-FIP2. We have found that gastric microsomal membrane extracts phosphorylate Rab11-FIP2 on serine 227. We identified the kinase that phosphorylated Rab11-FIP2 as MARK2/EMK1/Par-1Balpha (MARK2), and recombinant MARK2 phosphorylated Rab11-FIP2 only on serine 227. We created stable Madin-Darby canine kidney (MDCK) cell lines expressing enhanced green fluorescent protein-Rab11-FIP2 wild type or a nonphosphorylatable mutant [Rab11-FIP2(S227A)]. Analysis of these cell lines demonstrates a new role for Rab11-FIP2 in addition to that in the plasma membrane recycling system. In calcium switch assays, cells expressing Rab11-FIP2(S227A) showed a defect in the timely reestablishment of p120-containing junctional complexes. However, Rab11-FIP2(S227A) did not affect localization with recycling system components or the normal function of apical recycling and transcytosis pathways. These results indicate that phosphorylation of Rab11-FIP2 on serine 227 by MARK2 regulates an alternative pathway modulating the establishment of epithelial polarity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.