ArticleMolecular biology of the cell2006
Regulated synthesis and functions of laminin 5 in polarized madin-darby canine kidney epithelial cells.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Actin-ring segment switching drives nonadhesive gap closure.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A Bioinspired Scaffold with Anti-Inflammatory Magnesium Hydroxide and Decellularized Extracellular Matrix for Renal Tissue Regeneration.ACS central science · 2019Article
- Assembly of the β4-Integrin Interactome Based on Proximal Biotinylation in the Presence and Absence of Heterodimerization.Molecular & cellular proteomics : MCP · 2019Article
- Laminins in Epithelial Cell Polarization: Old Questions in Search of New Answers.Cold Spring Harbor perspectives in biology · 2017Review
- Integrin α3β1 regulates kidney collecting duct development via TRAF6-dependent K63-linked polyubiquitination of Akt.Molecular biology of the cell · 2015Article
- Aberrant expression of laminin-332 promotes cell proliferation and cyst growth in ARPKD.American journal of physiology. Renal physiology · 2014Article
- Article
- Laminin 511 partners with laminin 332 to mediate directional migration of Madin-Darby canine kidney epithelial cells.Molecular biology of the cell · 2012Article
- The proton translocation domain of cellular vacuolar ATPase provides a target for the treatment of influenza A virus infections.British journal of pharmacology · 2011Article
- Regulation of Kir4.1 and AQP4 expression and stability at the basolateral domain of epithelial MDCK cells by the extracellular matrix.American journal of physiology. Renal physiology · 2011Article
- Autocrine transforming growth factor-{beta}1 activation mediated by integrin {alpha}V{beta}3 regulates transcriptional expression of laminin-332 in Madin-Darby canine kidney epithelial cells.Molecular biology of the cell · 2010Article
- Snail regulates cell-matrix adhesion by regulation of the expression of integrins and basement membrane proteins.The Journal of biological chemistry · 2008Article
- Roles of P21-activated kinases and associated proteins in epithelial wound healing.International review of cell and molecular biology · 2008Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Renal tubular epithelial cells synthesize laminin (LN)5 during regeneration of the epithelium after ischemic injury. LN5 is a truncated laminin isoform of particular importance in the epidermis, but it is also constitutively expressed in a number of other epithelia. To investigate the role of LN5 in morphogenesis of a simple renal epithelium, we examined the synthesis and function of LN5 in the spreading, proliferation, wound-edge migration, and apical-basal polarization of Madin-Darby canine kidney (MDCK) cells. MDCK cells synthesize LN5 only when subconfluent, and they degrade the existing LN5 matrix when confluent. Through the use of small-interfering RNA to knockdown the LN5 alpha3 subunit, we were able to demonstrate that LN5 is necessary for cell proliferation and efficient wound-edge migration, but not apical-basal polarization. Surprisingly, suppression of LN5 production caused cells to spread much more extensively than normal on uncoated surfaces, and exogenous keratinocyte LN5 was unable to rescue this phenotype. MDCK cells also synthesized laminin alpha5, a component of LN10, that independent studies suggest may form an assembled basal lamina important for polarization. Overall, our findings indicate that LN5 is likely to play an important role in regulating cell spreading, migration, and proliferation during reconstitution of a continuous epithelium.
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