ArticleWorld journal of gastroenterology2006
Apoptosis of human colon carcinoma HT-29 cells induced by ceramide.
Article in World journal of gastroenterology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 22 citations in OpenAlex.
- Egg-derived sphingomyelin induces ferroptosis in cancer cells, depending on intracellular labile iron pool levels.Biochemistry and biophysics reports · 2025Article
- Therapeutic Potential for Sphingolipids in Inflammatory Bowel Disease and Colorectal Cancer.Cancers · 2024Review
- Anti-microbial and anti-cancer efficacy of acetone extract of Rosa chinensis against resistant strain and lung cancer cell line.BMC complementary medicine and therapies · 2023Article
- RIPK1 and RIPK3 are positive prognosticators for cervical cancer patients and C2 ceramide can inhibit tumor cell proliferationFrontiers in oncology · 2023Article
- Article
- C2-ceramide induces cell death and protective autophagy in head and neck squamous cell carcinoma cells.International journal of molecular sciences · 2014Article
- Deoxycholate, an endogenous cytotoxin/genotoxin, induces the autophagic stress-survival pathway: implications for colon carcinogenesis.Journal of toxicology · 2009Article
- Gli-1 siRNA induced apoptosis in Huh7 cells.World journal of gastroenterology · 2008Article
- Hydrophobic bile acids, genomic instability, Darwinian selection, and colon carcinogenesis.Clinical and experimental gastroenterology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate the effect of exogenous ceramide-induced apoptosis on human colon carcinoma HT-29 cells.
methodsLight microscope, transmission electron microscope and fluorescence microscope were used to observe the morphology change of apoptosis in HT-29 cells. Agarose gel electrophoresis was performed to detect the DNA fragment. Mitochondrial function was detected by MTT assay. mRNA expression of Bcl-2 family gene members was determined by reverse transcription polymerase chain reaction (RT-PCR) assay.
resultsAfter C2-ceramide treatment, typical characteristics of apoptosis, such as nuclear chromatin breakage, apoptotic body and DNA ladder, could be observed. After exposure to 50 micromol/L C2-ceramide for 12 and 24 h, cell apoptosis was 64.1% and 81.3% respectively, which had a time-and dose-effect relationship. Mitochondrial function started to decrease from 6 h after exposure to ceramide. Meanwhile, ceramide up-regulated or down-regulated the mRNA expression of Bcl-2 family gene members.
conclusionCeramide induces apoptosis of human colon carcinoma HT-29 cells by affecting the expression of Bcl-2 family gene members and impacting the mitochondrial function.
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Registered trials
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