ArticleNucleic acids research2006
The nucleolar protein Esf2 interacts directly with the DExD/H box RNA helicase, Dbp8, to stimulate ATP hydrolysis.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed, 65 citations in OpenAlex.
- Annotation and functional prediction of RNA helicases inIMA fungus · 2025Review
- Identification of two transcription factors that work coordinately to regulate early development inmBio · 2024Article
- Ribosome biogenesis factors-from names to functions.The EMBO journal · 2023Review
- ABT1 modifies SMARD1 pathology via interactions with IGHMBP2 and stimulation of ATPase and helicase activity.JCI insight · 2023Article
- RNA folding and functions of RNA helicases in ribosome biogenesis.RNA biology · 2022Review
- The SSU Processome Component Utp25p is a Pseudohelicase.microPublication biology · 2022Article
- Sgd1 is an MIF4G domain-containing cofactor of the RNA helicase Fal1 and associates with the 5' domain of the 18S rRNA sequence.RNA biology · 2020Article
- Assembly of the small ribosomal subunit in yeast: mechanism and regulation.RNA (New York, N.Y.) · 2018Review
- Maturation of the 90S pre-ribosome requires Mrd1 dependent U3 snoRNA and 35S pre-rRNA structural rearrangements.Nucleic acids research · 2018Article
- Recruitment, Duplex Unwinding and Protein-Mediated Inhibition of the Dead-Box RNA Helicase Dbp2 at Actively Transcribed Chromatin.Journal of molecular biology · 2016Article
- Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways.RNA biology · 2016Article
- Stage-specific assembly events of the 6-MDa small-subunit processome initiate eukaryotic ribosome biogenesis.Nature structural & molecular biology · 2015Article
- Measuring helicase inhibition of the DEAD-box protein Dbp2 by Yra1.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Regulation of DEAH/RHA helicases by G-patch proteins.BioMed research international · 2015Review
- Physical and functional interaction between the methyltransferase Bud23 and the essential DEAH-box RNA helicase Ecm16.Molecular and cellular biology · 2014Article
- Ribosome biogenesis in the yeast Saccharomyces cerevisiae.Genetics · 2013Review
- The DEAD-box protein Dbp2 functions with the RNA-binding protein Yra1 to promote mRNP assembly.Journal of molecular biology · 2013Article
- Cofactor-dependent specificity of a DEAD-box protein.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Article
- The DEAD-box helicase eIF4A: paradigm or the odd one out?RNA biology · 2013Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
While 18 putative RNA helicases are involved in ribosome biogenesis in Saccharomyces cerevisiae, their enzymatic properties have remained largely biochemically uncharacterized. To better understand their function, we examined the enzymatic properties of Dpb8, a DExD/H box protein previously shown to be required for the synthesis of the 18S rRNA. As expected for an RNA helicase, we demonstrate that recombinant Dbp8 has ATPase activity in vitro, and that this activity is dependent on an intact ATPase domain. Strikingly, we identify Esf2, a nucleolar putative RNA binding protein, as a binding partner for Dbp8, and show that it enhances Dbp8 ATPase activity by decreasing the K(M) for ATP. Thus, we have uncovered Esf2 as the first example of a protein co-factor that has a stimulatory effect on a nucleolar RNA helicase. We show that Esf2 can bind to pre-rRNAs and speculate that it may function to bring Dbp8 to the pre-rRNA, thereby both regulating its enzymatic activity and guiding Dbp8 to its site of action.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.