ArticleBMC infectious diseases2006
Usefulness of real time PCR for the differentiation and quantification of 652 and JP2 Actinobacillus actinomycetemcomitans genotypes in dental plaque and saliva.
Article in BMC infectious diseases, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 43 citations in OpenAlex.
- Prevalence of the JP2 genotype of Aggregatibacter actinomycetemcomitans in the world population: a systematic review.Clinical oral investigations · 2022Pooled it
- Periodontal health in teenagers treated with removable aligners and fixed orthodontic appliances.Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie · 2015Trial
- Occurrence ofMicroorganisms · 2024Article
- Carriage of the JP2 Genotype ofPathogens (Basel, Switzerland) · 2022Article
- Changes in the oral status and periodontal pathogens in a Sardinian rural community from pre-industrial to modern time.Scientific reports · 2022Article
- Prevalence of JP2 and Non-JP2 Genotypes ofPathogens (Basel, Switzerland) · 2021Article
- Prevalence ofPathogens (Basel, Switzerland) · 2020Article
- The Best Peptidomimetic Strategies to Undercover Antibacterial Peptides.International journal of molecular sciences · 2020Review
- Characterization ofVaccines · 2020Article
- Periodontal disease and influence of periodontal treatment on disease activity in patients with rheumatoid arthritis and spondyloarthritis.Rheumatology international · 2020Article
- Porphyromonas gingivalis in the tongue biofilm is associated with clinical outcome in rheumatoid arthritis patients.Clinical and experimental immunology · 2018Article
- Genetic Variants Involved in Bipolar Disorder, a Rough Road Ahead.Clinical practice and epidemiology in mental health : CP & EMH · 2018Article
- Design of FRET Probes for SNP RS1006737, Related to Mood Disorder.Clinical practice and epidemiology in mental health : CP & EMH · 2018Article
- Detection of a 640-bp deletion in the Aggregatibacter actinomycetemcomitans leukotoxin promoter region in isolates from an adolescent of Ethiopian origin.Journal of oral microbiology · 2015Article
- Article
- Pathogenicity of the highly leukotoxic JP2 clone of Aggregatibacter actinomycetemcomitans and its geographic dissemination and role in aggressive periodontitis.Journal of oral microbiology · 2014Review
- Oil Essential Mouthwashes Antibacterial Activity against Aggregatibacter actinomycetemcomitans: A Comparison between Antibiofilm and Antiplanktonic Effects.International journal of dentistry · 2013Article
- Microbial changes in subgingival plaque and polymicrobial intracellular flora in buccal cells after fixed orthodontic appliance therapy: a preliminary study.International journal of dentistry · 2013Article
- Quantitative discrimination of Aggregatibacter actinomycetemcomitans highly leukotoxic JP2 clone from non-JP2 clones in diagnosis of aggressive periodontitis.BMC infectious diseases · 2012Article
- Interactions of extracts from selected chewing stick sources with Aggregatibacter actinomycetemcomitans.BMC research notes · 2012Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe aim of our study is to describe a fast molecular method, able to distinguish and quantize the two different genotypes (652 and JP2) of an important periodontal pathogen: Actinobacillus actinomycetemcomitans. The two genotypes show differences in the expression of an important pathogenic factor: the leukotoxin (ltx). In order to evidence this, we performed a real time PCR procedure on the ltx operon, able to recognize Aa clinical isolates with different leukotoxic potentials.
methodsThe specificity of the method was confirmed in subgingival plaque and saliva specimens collected from eighty-one Italian (Sardinian) subjects with a mean age of 43.9, fifty five (68 %) of whom had various clinical forms of periodontal disease.
resultsThis procedure showed a good sensitivity and a high linear dynamic range of quantization (10(7)-10(2) cells/ml) for all genotypes and a good correlation factor (R2 = 0.97-0.98). Compared with traditional cultural methods, this real time PCR procedure is more sensitive; in fact in two subgingival plaque and two positive saliva specimens Aa was only detected with the molecular method.
conclusionA low number of Sardinian patients was found positive for Aa infections in the oral cavity, (just 10 positive periodontal cases out of 81 and two of these were also saliva positive). The highly leukotoxic JP2 strain was the most representative (60 % of the positive specimens); the samples from periodontal pockets and from saliva showed some ltx genotype for the same patient. Our experience suggests that this approach is suitable for a rapid and complete laboratory diagnosis for Aa infection.
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