ArticleCurrent genetics2006
Insertion mutagenesis of the yeast Candida famata (Debaryomyces hansenii) by random integration of linear DNA fragments.
Article in Current genetics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 58 citations in OpenAlex.
- Hog1 MAP kinase modulates early riboflavin accumulation under low-pH and saline conditions inFrontiers in microbiology · 2026Article
- Regulation of Riboflavin Biosynthesis in Microorganisms and Construction of the Advanced Overproducers of This Vitamin.International journal of molecular sciences · 2025Review
- Lignocellulosic biomass fermentation: a roadmap for Candida famata and Ogataea polymorpha.FEMS yeast research · 2025Review
- The Sef1 transcription factor interacts with promoters of riboflavin structural genes in Candida famata.FEMS yeast research · 2025Article
- Metabolic engineering of non-conventional yeasts for construction of the advanced producers of biofuels and high-value chemicals.BBA advances · 2023Article
- Genome Sequence and Analysis of the Flavinogenic YeastJournal of fungi (Basel, Switzerland) · 2022Article
- Molecular Elucidation of Riboflavin Production and Regulation in Candida albicans, toward a Novel Antifungal Drug Target.mSphere · 2020Article
- History of genome editing in yeast.Yeast (Chichester, England) · 2018Review
- New kids on the block: emerging oleaginous yeast of biotechnological importance.AIMS microbiology · 2017Review
- Metabolic and bioprocess engineering of the yeast Candida famata for FAD production.Journal of industrial microbiology & biotechnology · 2014Article
- Identification of yeast associated with the planthopper, Perkinsiella saccharicida: potential applications for Fiji leaf gall control.Current microbiology · 2011Article
- Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers.Microbiology and molecular biology reviews : MMBR · 2011Review
- Identification of the genes affecting the regulation of riboflavin synthesis in the flavinogenic yeast Pichia guilliermondii using insertion mutagenesis.FEMS yeast research · 2011Article
- Deficiency in frataxin homologue YFH1 in the yeast Pichia guilliermondii leads to missregulation of iron acquisition and riboflavin biosynthesis and affects sulfate assimilation.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The feasibility of using random insertional mutagenesis to isolate mutants of the flavinogenic yeast Candida famata was explored. Mutagenesis was performed by transformation of the yeast with an integrative plasmid containing the Saccharomyces cerevisiae LEU2 gene as a selective marker. The addition of restriction enzyme together with the plasmid (restriction enzyme-mediated integration, REMI) increased the transformation frequency only slightly. Integration of the linearized plasmid occurred randomly in the C. famata genome. To investigate the potential of insertional mutagenesis, it was used for tagging genes involved in positive regulation of riboflavin synthesis in C. famata. Partial DNA sequencing of tagged genes showed that they were homologous to the S. cerevisiae genes RIB1, MET2, and SEF1. Intact orthologs of these genes isolated from Debaryomyces hansenii restored the wild phenotype of the corresponding mutants, i.e., the ability to overproduce riboflavin under iron limitation. The Staphylococcus aureus ble gene conferring resistance to phleomycin was used successfully in the study as a dominant selection marker for C. famata. The results obtained indicate that insertional mutagenesis is a powerful tool for tagging genes in C. famata.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.