ArticleHistochemistry and cell biology2006
Antibody-specific detection of caveolin-1 in subapical compartments of MDCK cells.
Article in Histochemistry and cell biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Caveolar and non-Caveolar Caveolin-1 in ocular homeostasis and disease.Progress in retinal and eye research · 2022Review
- Caveolin-1α regulates primary cilium length by controlling RhoA GTPase activity.Scientific reports · 2019Article
- Characterization of a caveolin-1 mutation associated with both pulmonary arterial hypertension and congenital generalized lipodystrophy.Traffic (Copenhagen, Denmark) · 2016Article
- Cytoskeleton modification and cholesterol depletion affect membrane properties and caveolae positioning of CHO cells.The Journal of membrane biology · 2014Article
- Coordinated regulation of caveolin-1 and Rab11a in apical recycling compartments of polarized epithelial cells.Experimental cell research · 2012Article
- Targeting and imaging single biomolecules in living cells by complementation-activated light microscopy with split-fluorescent proteins.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Is caveolin involved in normal proximal tubule function? Presence in model PT systems but absence in situ.American journal of physiology. Renal physiology · 2011Article
- Tyrosine-phosphorylated caveolin-1 blocks bacterial uptake by inducing Vav2-RhoA-mediated cytoskeletal rearrangements.PLoS biology · 2010Article
- Caveolin-1 and -2 interact with connexin43 and regulate gap junctional intercellular communication in keratinocytes.Molecular biology of the cell · 2008Article
- Recent progress in histochemistry.Histochemistry and cell biology · 2007Review
- Cholesterol-sensitive modulation of transcytosis.Molecular biology of the cell · 2007Article
- The histochemistry and cell biology vade mecum: a review of 2005-2006.Histochemistry and cell biology · 2006Review
- Caveolae, ion channels and cardiac arrhythmias.Progress in biophysics and molecular biologyReview
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Caveolin-1 is the major structural component of caveolae and is also found in the Golgi complex of many cell types. Occasionally, caveolin-1 has been observed in additional intracellular compartments, including recycling endosomes. Why caveolin-1 expression is detected at these sites only infrequently is not clear. In this study, we test the hypothesis that non-caveolar, non-Golgi pools of caveolin-1 display unique and/or fixation-dependent epitopes. We compared the ability of a panel of antibodies raised against various domains of caveolin-1 to detect distinct subcellular pools of the protein by immunofluorescence microscopy in Madin-Darby canine kidney (MDCK) cells, a cell line where the subcellular localization of caveolin-1 has been extensively characterized. We show that three antibodies directed to the N-terminus of caveolin-1 recognize a previously undetected pool of caveolin-1 in the subapical region of MDCK cells, a localization characteristic of endosomal recycling compartments. The antibodies vary in their ability to label caveolin-1 at the cell surface, and the epitopes detected by each are highly fixation dependent. Our findings suggest that no single caveolin antibody or staining condition is capable of detecting all the caveolin-1 in a cell simultaneously. Consequently, the subcellular distribution of caveolin-1 may be much broader than currently believed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.