ArticleNucleic acids research2006
Pyrene binary probes for unambiguous detection of mRNA using time-resolved fluorescence spectroscopy.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- A water-soluble pyrene-based diimidazolium molecular probe enabling chirality and fluorescence dual-response toward G-quadruplexes.Chemical science · 2026Article
- Unveiling the Light Induced Energy Transfer Events in Water Soluble Benzothiazole Appended Borondipyrromethene: Synthesis, Photophysical Study, and Applications to Amyloid Binding.Chemistry, an Asian journal · 2026Article
- Awareness for artifacts in fluorescence microscopy of β-TCP.BMC research notes · 2024Article
- Recent Advances in Excimer-Based Fluorescence Probes for Biological Applications.Molecules (Basel, Switzerland) · 2022Review
- DNA/RNA Fluorescence Imaging by Synthetic Nucleic Acids.Advances in experimental medicine and biology · 2021Article
- A robust photoluminescence screening assay identifies uracil-DNA glycosylase inhibitors against prostate cancer.Chemical science · 2020Article
- Interaction of Cucurbit[7]uril With Protease Substrates: Application to Nanosecond Time-Resolved Fluorescence Assays.Frontiers in chemistry · 2020Article
- Hydrophilic, Red-Emitting, and Thermally Activated Delayed Fluorescence Emitter for Time-Resolved Luminescence Imaging by Mitochondrion-Induced Aggregation in Living Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- Recent Advances in Nucleic Acid Targeting Probes and Supramolecular Constructs Based on Pyrene-Modified Oligonucleotides.Molecules (Basel, Switzerland) · 2017Review
- Fluorescent probes for nucleic Acid visualization in fixed and live cells.Molecules (Basel, Switzerland) · 2013Review
- Multipyrene tandem probes for point mutations detection in DNA.Journal of nucleic acids · 2013Article
- DNA-multichromophore systems.Chemical reviews · 2012Review
- Fluorescent hybridization probes for nucleic acid detection.Analytical and bioanalytical chemistry · 2012Review
- Mercury(II) ion detection via pyrene-mediated photolysis of disulfide bonds.Chemistry (Weinheim an der Bergstrasse, Germany) · 2012Article
- Pyrene-functionalized oligonucleotides and locked nucleic acids (LNAs): tools for fundamental research, diagnostics, and nanotechnology.Chemical Society reviews · 2011Review
- Nucleic acid-based fluorescent probes and their analytical potential.Analytical and bioanalytical chemistry · 2011Review
- Competition-mediated pyrene-switching aptasensor: probing lysozyme in human serum with a monomer-excimer fluorescence switch.Analytical chemistry · 2010Article
- The fluorescence properties and lifetime study of G-quadruplexes single- and double-labeled with pyrene.Journal of fluorescence · 2010Article
- The concept of λ-ratiometry in fluorescence sensing and imaging.Journal of fluorescence · 2010Review
- Telomeric repeat-containing RNA structure in living cells.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
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Authors and funding
11 authors.
Funding
Abstract
We report here the design, synthesis and application of pyrene binary oligonucleotide probes for selective detection of cellular mRNA. The detection strategy is based on the formation of a fluorescent excimer when two pyrene groups are brought into close proximity upon hybridization of the probes with the target mRNA. The pyrene excimer has a long fluorescence lifetime (>40 ns) compared with that of cellular extracts (approximately 7 ns), allowing selective detection of the excimer using time-resolved emission spectra (TRES). Optimized probes were used to target a specific region of sensorin mRNA yielding a strong excimer emission peak at 485 nm in the presence of the target and no excimer emission in the absence of the target in buffer solution. While direct fluorescence measurement of neuronal extracts showed a strong fluorescent background, obscuring the detection of the excimer signal, time-resolved emission measurements indicated that the emission decay of the cellular extracts is approximately 8 times faster than that of the pyrene excimer probes. Thus, using TRES of the pyrene probes, we are able to selectively detect mRNA in the presence of cellular extracts, demonstrating the potential for application of pyrene excimer probes for imaging mRNAs in cellular environments that have background fluorescence.
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Registered trials
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