ArticleParasitology research2006
Long-term storage of Clonorchis sinensis metacercariae in vitro.
Article in Parasitology research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 15 citations in OpenAlex.
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- Efficacy assessment of miltefosine and curcumin againstAntimicrobial agents and chemotherapy · 2024Article
- Analysis of the population genetics of Opisthorchis viverrini sensu lato in the Nam Ngum River wetland, Lao PDR, by multilocus enzyme electrophoresis.Parasitology research · 2014Article
- Survival of heterophyid metacercaria in common carp (Cyprinus carpio).Parasitology research · 2013Article
- Early detection and estimation of infection burden by real-time PCR in rats experimentally infected with Clonorchis sinensis.Parasitology research · 2011Article
- Factors in the resistance of rats to re-infection and super-infection by Clonorchis sinensis.Parasitology research · 2008Article
- Changing patterns of serum and bile antibodies in re-infected rats with Clonorchis sinensis.The Korean journal of parasitology · 2008Article
- Association of bacteria with metacercarial cysts of Echinostoma caproni.Parasitology research · 2007Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clonorchis sinensis metacercariae must often be stored for several months to meet laboratory requirements. In the present study, the authors evaluated morphological changes and infectivities of metacercariae kept in different storage solutions for 1, 3, and 6 months, i.e., PBS (phosphate buffered saline), PBS + antibiotics, PBS + iodoacetic acid (IAA), and PBS + antibiotics + IAA. Metacercariae stored in PBS containing antibiotics showed significantly higher survival rates and infectivities than those stored in PBS alone after 3 or 6 months. The metacercariae that had been stored for 6 months in solutions that were refreshed monthly had also significantly higher survival and infection rates than those stored in nonrefreshed solutions. Moreover, the addition of IAA, an inhibitor of cysteine proteinase, to these storage solutions reduced the survival and infectivity. The results of the present study confirm that the viabilities of C. sinensis metacercariae are best maintained in storage solutions containing antibiotics that are regularly refreshed.
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Registered trials
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