ArticleDevelopmental biology2006
Dose-dependent Smad1, Smad5 and Smad8 signaling in the early mouse embryo.
Article in Developmental biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
80 citing papers in PubMed, 157 citations in OpenAlex.
- FKBP12 determines ligand-specific SMAD signaling in individual living endothelial cells.iScience · 2026Article
- Optogenetic BMP4 models symmetry breaking and primitive streak formation in hPSCs.bioRxiv : the preprint server for biology · 2026Article
- Dampened HPG axis activity and altered ovarian gene transcription in Dummerstorf high-fertility mouse line FL2.Endocrine connections · 2026Article
- High resolution spatial transcriptomic and proteomic profiling of early primate gastrulationbioRxiv : the preprint server for biology · 2026Article
- Translational biomarkers of hypoxic brain injury uncovered in CSF secreting human choroid plexus organoids.Fluids and barriers of the CNS · 2025Article
- Molecular Determinants of Bone Plasticity Regeneration After Trauma: Forensic Consequences.International journal of molecular sciences · 2025Review
- Review
- Potential Candidate Genes Associated with Litter Size in Goats: A Review.Animals : an open access journal from MDPI · 2025Review
- Loss of TJP1 disrupts gastrulation patterning and increases differentiation toward the germ cell lineage in human pluripotent stem cells.Developmental cell · 2023Article
- BMP4 triggers regulatory circuits specifying the cardiac mesoderm lineage.Development (Cambridge, England) · 2023Article
- Review
- The versatility and paradox of BMP signaling in endothelial cell behaviors and blood vessel function.Cellular and molecular life sciences : CMLS · 2022Review
- SMAD1 Loss-of-Function Variant Responsible for Congenital Heart Disease.BioMed research international · 2022Article
- ASB2 is a novel E3 ligase of SMAD9 required for cardiogenesis.Scientific reports · 2021Article
- A comparative analysis of heart microRNAs in vertebrates brings novel insights into the evolution of genetic regulatory networks.BMC genomics · 2021Article
- Step by Step about Germ Cells Development in Canine.Animals : an open access journal from MDPI · 2021Review
- Review
- Intracellular Communication among Morphogen Signaling Pathways during Vertebrate Body Plan Formation.Genes · 2020Review
- ALK3-SMAD1/5 Signaling Mediates the BMP2-Induced Decrease in PGE2 Production in Human Endometrial Stromal Cells and Decidual Stromal Cells.Frontiers in cell and developmental biology · 2020Article
- PRDM15 loss of function links NOTCH and WNT/PCP signaling to patterning defects in holoprosencephaly.Science advances · 2020Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 2 countries.
Funding
Abstract
Three closely related mammalian R-Smads, namely Smad1, Smad5 and Smad8, are activated by BMP receptors. Here we have taken a genetic approach to further dissect their possibly unique and/or shared roles during early mouse development. A Smad8.LacZ reporter allele was created to visualize Smad8 expression domains. Smad8 is initially expressed only in the visceral yolk sac (VYS) endoderm and shows a highly restricted pattern of expression in the embryo proper at later stages. In addition, Smad8 conditional and null alleles were engineered. All alleles clearly demonstrate that adult Smad8 homozygous mutants are viable and fertile. To elucidate gene dosage effects, we manipulated expression ratios of the three BMP R-Smads. Smad8 homozygotes also lacking one copy of Smad1 or Smad5 did not exhibit overt phenotypes, and the tissue disturbances seen in Smad1 or Smad5 null embryos were not exacerbated in the absence of Smad8. However, we discovered a profound genetic interaction between Smad1 and Smad5. Thus, as for Smad1 and Smad5 mutant embryos, Smad1+/-:Smad5+/- double heterozygotes die by E10.5 and display defects in allantois morphogenesis, cardiac looping and primordial germ cell (PGC) specification. These experiments demonstrate for the first time that Smad1 and Smad5 function cooperatively to govern BMP target gene expression in the early mammalian embryo.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.