ArticleRetrovirology2006
Interactions between Nef and AIP1 proliferate multivesicular bodies and facilitate egress of HIV-1.
Article in Retrovirology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.
- HIV Genome-Wide Protein Associations: a Review of 30 Years of Research.Microbiology and molecular biology reviews : MMBR · 2016Pooled it
- Review
- HIV protein Nef expression in human microglia drives the release of distinct Nef-containing extracellular vesicles.Extracellular vesicles and circulating nucleic acids · 2025Article
- Role of Monocyte/Macrophages in the Pathogenesis of NeuroHIV.Results and problems in cell differentiation · 2024Review
- HIV-1 Nef Protein Affects Cytokine and Extracellular Vesicles Production in the GEN2.2 Plasmacytoid Dendritic Cell Line.Viruses · 2021Article
- How HIV Nef Proteins Hijack Membrane Traffic To Promote Infection.Journal of virology · 2019Review
- Slow Release of HIV-1 Protein Nef from Vesicle-like Structures Is Inhibited by Cytosolic Calcium Elevation in Single Human Microglia.Molecular neurobiology · 2019Article
- The Ambiguous Roles of Extracellular Vesicles in HIV Replication and Pathogenesis.Frontiers in microbiology · 2018Review
- Exosomes Derived from HIV-1 Infected DCs Mediate Viral trans-Infection via Fibronectin and Galectin-3.Scientific reports · 2017Article
- Nef is secreted in exosomes from Nef.GFP-expressing and HIV-1-infected human astrocytes.Journal of neurovirology · 2017Article
- Enterovirus 71 transmission by exosomes establishes a productive infection in human neuroblastoma cells.Virus genes · 2016Article
- Exosomes: Implications in HIV-1 Pathogenesis.Viruses · 2015Review
- A Conserved GPG-Motif in the HIV-1 Nef Core Is Required for Principal Nef-Activities.PloS one · 2015Article
- Interaction of HIV-1 Nef protein with the host protein Alix promotes lysosomal targeting of CD4 receptor.The Journal of biological chemistry · 2014Article
- The HIV Nef protein modulates cellular and exosomal miRNA profiles in human monocytic cells.Journal of extracellular vesicles · 2014Article
- Nef neutralizes the ability of exosomes from CD4+ T cells to act as decoys during HIV-1 infection.PloS one · 2014Article
- HIV-1 Nef inhibits Protease activity and its absence alters protein content of mature viral particles.PloS one · 2014Article
- The frantic play of the concealed HIV envelope cytoplasmic tail.Retrovirology · 2013Review
- Comparative proteomic analysis of HIV-1 particles reveals a role for Ezrin and EHD4 in the Nef-dependent increase of virus infectivity.Journal of virology · 2013Article
- The HIV-1 Nef protein binds argonaute-2 and functions as a viral suppressor of RNA interference.PloS one · 2013Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundNef is an accessory protein of primate lentiviruses, HIV-1, HIV-2 and SIV. Besides removing CD4 and MHC class I from the surface and activating cellular signaling cascades, Nef also binds GagPol during late stages of the viral replicative cycle. In this report, we investigated further the ability of Nef to facilitate the replication of HIV-1.
resultsTo this end, first the release of new viral particles was much lower in the absence of Nef in a T cell line. Since the same results were obtained in the absence of the viral envelope using pseudo-typed viruses, this phenomenon was independent of CD4 and enhanced infectivity. Next, we found that Nef not only possesses a consensus motif for but also binds AIP1 in vitro and in vivo. AIP1 is the critical intermediate in the formation of multivesicular bodies (MVBs), which play an important role in the budding and release of viruses from infected cells. Indeed, Nef proliferated MVBs in cells, but only when its AIP1-binding site was intact. Finally, these functions of Nef were reproduced in primary macrophages, where the wild type but not mutant Nef proteins led to increased release of new viral particles from infected cells.
conclusionWe conclude that by binding GagPol and AIP1, Nef not only proliferates MVBs but also contributes to the egress of viral particles from infected cells.
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Registered trials
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