ArticleProceedings of the National Academy of Sciences of the United States of America2006
Identification of bifunctional delta12/omega3 fatty acid desaturases for improving the ratio of omega3 to omega6 fatty acids in microbes and plants.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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62 citing papers in PubMed, 174 citations in OpenAlex.
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- Molecular cloning and functional analysis of a plastidial ω3 desaturase fromFrontiers in microbiology · 2024Article
- A combined transcriptomic and physiological approach to understanding the adaptive mechanisms to cope with oxidative stress inMicrobiology spectrum · 2023Article
- Biomanufacturing of γ-linolenic acid-enriched galactosyldiacylglycerols: Challenges in microalgae and potential in oleaginous yeasts.Synthetic and systems biotechnology · 2023Review
- Bioengineering of Soybean Oil and Its Impact on Agronomic Traits.International journal of molecular sciences · 2023Review
- EngineeringSynthetic and systems biotechnology · 2022Review
- Fatty acid desaturases (FADs) modulate multiple lipid metabolism pathways to improve plant resistance.Molecular biology reports · 2022Review
- Microbes: A Hidden Treasure of Polyunsaturated Fatty Acids.Frontiers in nutrition · 2022Review
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- Characterization of fatty acid desaturases reveals stress-induced synthesis of C18 unsaturated fatty acids enriched in triacylglycerol in the oleaginous alga Chromochloris zofingiensis.Biotechnology for biofuels · 2021Article
- Understanding and exploiting the fatty acid desaturation system in Rhodotorula toruloides.Biotechnology for biofuels · 2021Article
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- Lipid metabolism of the oleaginous yeast Lipomyces starkeyi.Applied microbiology and biotechnology · 2020Review
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- Overexpression of △12, △15-Desaturases for Enhanced Lipids Synthesis inFrontiers in microbiology · 2020Article
- Generation ofOpen biology · 2019Article
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2 more citing papers are in PubMed but not listed here.
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7 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
We report the identification of bifunctional Delta12/omega3 desaturases from Fusarium moniliforme, Fusarium graminearum, and Magnaporthe grisea. The bifunctional activity of these desaturases distinguishes them from all known Delta12 or omega3 fatty acid desaturases. The omega3 desaturase activity of these enzymes also shows a broad omega6 fatty acid substrate specificity by their ability to convert linoleic acid (LA), gamma-linolenic acid, di-homo-gamma-linolenic acid, and arachidonic acid to the omega3 fatty acids, alpha-linolenic acid (ALA), stearidonic acid, eicosatetraenoic acid, and eicosapentaenoic acid (EPA), respectively. Phylogenetic analysis suggests that omega3 desaturases arose by independent gene duplication events from a Delta12 desaturase ancestor. Expression of F. moniliforme Delta12/omega3 desaturase resulted in high ALA content in both Yarrowia lipolytica, an oleaginous yeast naturally deficient in omega3 desaturation, and soybean. In soybean, seed-specific expression resulted in 70.9 weight percent of total fatty acid (%TFA) ALA in a transformed seed compared with 10.9%TFA in a null segregant seed and 53.2%TFA in the current best source of ALA, linseed oil. The ALA/LA ratio in transformed seed was 22.3, a 110- and 7-fold improvement over the null segregant seed and linseed oil, respectively. Thus, these desaturases have potential for producing nutritionally desirable omega3 long-chain polyunsaturated fatty acids, such as EPA, with a significantly improved ratio of omega3/omega6 long-chain polyunsaturated fatty acids in both oilseeds and oleaginous microbes.
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