ArticleBiosensors & bioelectronics2007
DNA hybridization sensor based on aurothiomalate electroactive label on glassy carbon electrodes.
Article in Biosensors & bioelectronics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 33 citations in OpenAlex.
- Nanomaterial-based sensors for microbe detection: a review.Discover nano · 2024Review
- Perspectives on electrochemical biosensing of COVID-19.Current opinion in electrochemistry · 2021Review
- Emerging materials for the electrochemical detection of COVID-19.Journal of electroanalytical chemistry (Lausanne, Switzerland) · 2021Review
- The potential of electrochemistry for the detection of coronavirus-induced infections.Trends in analytical chemistry : TRAC · 2020Review
- Nanobiosensors for the Detection of Novel Coronavirus 2019-nCoV and Other Pandemic/Epidemic Respiratory Viruses: A Review.Sensors (Basel, Switzerland) · 2020Review
- SPR Detection and Discrimination of the Oligonucleotides Related to the Normal and the Hybrid bcr-abl Genes by Two Stringency Control Strategies.Nanoscale research letters · 2016Article
- Methylene blue covalently attached to single stranded DNA as electroactive label for potential bioassays.Sensors and actuators. B, Chemical · 2014Article
- An electrochemical DNA microbiosensor based on succinimide-modified acrylic microspheres.Sensors (Basel, Switzerland) · 2012Article
- State-of-the-art of (bio)chemical sensor developments in analytical Spanish groups.Sensors (Basel, Switzerland) · 2010Review
- DNA hybridization biosensors using polylysine modified SPCEs.Biosensors & bioelectronics · 2008Article
- Electrochemical Sensors for Clinic Analysis.Sensors (Basel, Switzerland) · 2008Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this work, a gold complex is used as electroactive label for monitoring hybridization assays on glassy carbon electrodes. Ionic gold is bound to a 30-mer sequence of the SARS (severe acute respiratory syndrome) virus, responsible for the atypical pneumonia, using sodium aurothiomalate. In order to label this single strand, a mixture of sodium aurothiomalate and the strand is prepared. Then, it is incubated for 24 h at 37 degrees C and, finally, free gold is separated from the labeled strand by a dialysis against a 0.15M NaCl solution (pH 7.5). The DNA hybridization sensor is designed immobilizing the complementary probe on the pre-treated electrode surface and, then, the hybridization reaction takes place with the gold labeled strand. The electrochemical determination is based on the catalytic effect of electrodeposited gold on the reduction of silver ions. In non-stringent experimental conditions, a limit of detection of 15 fmol (30 microL) is obtained, and discrimination between a complementary oligonucleotide and a three-based mismatch complementary oligonucleotide is achieved. For the discrimination of a single-base mismatch, is needed to use stringent conditions (50% of formamide in the hybridization buffer).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.