ArticlePlant physiology2006
Expression and characterization of a redox-sensing green fluorescent protein (reduction-oxidation-sensitive green fluorescent protein) in Arabidopsis.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 157 citations in OpenAlex.
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- Chloroplast redox state changes mark cell-to-cell signaling in the hypersensitive response.The New phytologist · 2023Article
- Nanosensor Applications in Plant Science.Biosensors · 2022Review
- Systematic monitoring of 2-Cys peroxiredoxin-derived redox signals unveiled its role in attenuating carbon assimilation rate.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Intertwined Roles of Reactive Oxygen Species and Salicylic Acid Signaling Are Crucial for the Plant Response to Biotic Stress.International journal of molecular sciences · 2022Review
- Sensing stress responses in potato with whole-plant redox imaging.Plant physiology · 2021Article
- Deciphering the Role of Ion Channels in Early Defense Signaling against Herbivorous Insects.Cells · 2021Review
- Resolving diurnal dynamics of the chloroplastic glutathione redox state in Arabidopsis reveals its photosynthetically derived oxidation.The Plant cell · 2021Article
- A systemic whole-plant change in redox levels accompanies the rapid systemic response to wounding.Plant physiology · 2021Article
- Live monitoring of plant redox and energy physiology with genetically encoded biosensors.Plant physiology · 2021Review
- Chloroplast-derived photo-oxidative stress causes changes in H2O2 and EGSH in other subcellular compartments.Plant physiology · 2021Article
- Distinctive in-planta acclimation responses to basal growth and acute heat stress were induced in Arabidopsis by cattle manure biochar.Scientific reports · 2021Article
- Biosensors: A Sneak Peek into Plant Cell's Immunity.Life (Basel, Switzerland) · 2021Review
- Morphological, Physiological and Molecular Markers for Salt-Stressed Plants.Plants (Basel, Switzerland) · 2021Review
- Focus on biosensors: Looking through the lens of quantitative biology.Quantitative plant biology · 2021Review
- In Vivo Imaging with Genetically Encoded Redox Biosensors.International journal of molecular sciences · 2020Review
- Compensation of Mutation inInternational journal of molecular sciences · 2020Article
- Metabolic and oncogenic adaptations to pyruvate dehydrogenase inactivation in fibroblasts.The Journal of biological chemistry · 2019Article
- Forward genetic screens identify a role for the mitochondrial HER2 in E-2-hexenal responsiveness.Plant molecular biology · 2017Article
- Mitochondrially targeted fluorescent redox sensors.Interface focus · 2017Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arabidopsis (Arabidopsis thaliana) was transformed with a redox-sensing green fluorescent protein (reduction-oxidation-sensitive green fluorescent protein [roGFP]), with expression targeted to either the cytoplasm or to the mitochondria. Both the mitochondrial and cytosolic forms are oxidation-reduction sensitive, as indicated by a change in the ratio of 510 nm light (green light) emitted following alternating illumination with 410 and 474 nm light. The 410/474 fluorescence ratio is related to the redox potential (in millivolts) of the organelle, cell, or tissue. Both forms of roGFP can be reduced with dithiothreitol and oxidized with hydrogen peroxide. The average resting redox potentials for roots are -318 mV for the cytoplasm and -362 mV for the mitochondria. The elongation zone of the Arabidopsis root has a more oxidized redox status than either the root cap or meristem. Mitochondria are much better than the cytoplasm, as a whole, at buffering changes in redox. The data show that roGFP is redox sensitive in plant cells and that this sensor makes it possible to monitor, in real time, dynamic changes in redox in vivo.
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