ArticleMolecular biology of the cell2006
Stable CpG hypomethylation of adipogenic promoters in freshly isolated, cultured, and differentiated mesenchymal stem cells from adipose tissue.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
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Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it, 154 citations in OpenAlex.
- Sexual dimorphism of leptin and adiposity in children between 0 and 10 years: a systematic review and meta-analysis.Biology of sex differences · 2022Pooled it
- Exploring NamiRNA networks and time-series gene expression in osteogenic differentiation of adipose-derived stem cells.Annals of medicine · 2025Article
- Placenta DNA methylation levels of the promoter region of the leptin receptor gene are associated with infant cortisol.Psychoneuroendocrinology · 2023Article
- DNA methylation analysis identifies key transcription factors involved in mesenchymal stem cell osteogenic differentiation.Biological research · 2023Article
- Temporal Modulation of DNA Methylation and Gene Expression in Monolayer and 3D Spheroids of Dental Pulp Stem Cells during Osteogenic Differentiation: A Comparative Study.Tissue engineering and regenerative medicine · 2022Article
- A Mixture of Endocrine Disrupting Chemicals Associated with Lower Birth Weight in Children Induces Adipogenesis and DNA Methylation Changes in Human Mesenchymal Stem Cells.International journal of molecular sciences · 2022Article
- Higher Expression of DNA (de)methylation-Related Genes Reduces Adipogenicity in Dental Pulp Stem Cells.Frontiers in cell and developmental biology · 2022Article
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- DNA methylation and demethylation link the properties of mesenchymal stem cells: Regeneration and immunomodulation.World journal of stem cells · 2020Review
- Milk-Derived Extracellular Vesicles in Inter-Organism, Cross-Species Communication and Drug Delivery.Proteomes · 2020Review
- Senile Osteoporosis: The Involvement of Differentiation and Senescence of Bone Marrow Stromal Cells.International journal of molecular sciences · 2020Review
- Concurrent EPA and DHA Supplementation Impairs Brown Adipogenesis of C2C12 Cells.Frontiers in genetics · 2020Article
- Molecular Insight into the Interaction between Epigenetics and Leptin in Metabolic Disorders.Nutrients · 2019Review
- Thy1 (CD90) expression is regulated by DNA methylation during adipogenesis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Micro-RNAS Regulate Metabolic Syndrome-induced Senescence in Porcine Adipose Tissue-derived Mesenchymal Stem Cells through the P16/MAPK Pathway.Cell transplantation · 2018Article
- Molecular Mechanisms of Transdifferentiation of Adipose-Derived Stem Cells into Neural Cells: Current Status and Perspectives.Stem cells international · 2018Review
- The metabolic syndrome modifies the mRNA expression profile of extracellular vesicles derived from porcine mesenchymal stem cells.Diabetology & metabolic syndrome · 2018Article
- On the origin and impact of mesenchymal stem cell heterogeneity: new insights and emerging tools for single cell analysis.European cells & materials · 2017Review
- TET-mediated hydroxymethylcytosine at the Pparγ locus is required for initiation of adipogenic differentiation.International journal of obesity (2005) · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mesenchymal stem cells from adipose tissue can differentiate into mesodermal lineages. Differentiation potential, however, varies between clones of adipose stem cells (ASCs), raising the hypothesis that epigenetic differences account for this variability. We report here a bisulfite sequencing analysis of CpG methylation of adipogenic (leptin [LEP], peroxisome proliferator-activated receptor gamma 2 [PPARG2], fatty acid-binding protein 4 [FABP4], and lipoprotein lipase [LPL]) promoters and of nonadipogenic (myogenin [MYOG], CD31, and GAPDH) loci in freshly isolated human ASCs and in cultured ASCs, in relation to gene expression and differentiation potential. Uncultured ASCs display hypomethylated adipogenic promoters, in contrast to myogenic and endothelial loci, which are methylated. Adipogenic promoters exhibit mosaic CpG methylation, on the basis of heterogeneous methylation between cells and of variation in the extent of methylation of a given CpG between donors, and both between and within clonal cell lines. DNA methylation reflects neither transcriptional status nor potential for gene expression upon differentiation. ASC culture preserves hypomethylation of adipogenic promoters; however, between- and within-clone mosaic methylation is detected. Adipogenic differentiation also maintains the overall CpG hypomethylation of LEP, PPARG2, FABP4, and LPL despite demethylation of specific CpGs and transcriptional induction. Furthermore, enhanced methylation at adipogenic loci in primary differentiated cells unrelated to adipogenesis argues for ASC specificity of the hypomethylated state of these loci. Therefore, mosaic hypomethylation of adipogenic promoters may constitute a molecular signature of ASCs, and DNA methylation does not seem to be a determinant of differentiation potential of these cells.
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