ArticleIn vitro cellular & developmental biology. Animal
Multiple initial culture conditions enhance the establishment of cell lines from primary ovarian cancer specimens.
Article in In vitro cellular & developmental biology. Animal. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Exploiting a living biobank to delineate mechanisms underlying disease-specific chromosome instability.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2023Review
- Cell Proteomic Footprinting: Advances in the Quality of Cellular and Cell-Derived Cancer Vaccines.Pharmaceutics · 2023Review
- Discovery and preclinical characterization of the antagonist anti-PD-L1 monoclonal antibody LY3300054.Journal for immunotherapy of cancer · 2018Article
- The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy.Clinical cancer research : an official journal of the American Association for Cancer Research · 2015Article
- Article
- Identification of a Titin-derived HLA-A1-presented peptide as a cross-reactive target for engineered MAGE A3-directed T cells.Science translational medicine · 2013Article
- Monoclonal TCR-redirected tumor cell killing.Nature medicine · 2012Article
- Primary human ovarian epithelial cancer cells broadly express HER2 at immunologically-detectable levels.PloS one · 2012Article
- Expression of a functional CCR2 receptor enhances tumor localization and tumor eradication by retargeted human T cells expressing a mesothelin-specific chimeric antibody receptor.Clinical cancer research : an official journal of the American Association for Cancer Research · 2011Article
- Article
- Cellular cancer vaccines: an update on the development of vaccines generated from cell surface antigens.Journal of Cancer · 2010Article
- Steric shielding of surface epitopes and impaired immune recognition induced by the ebola virus glycoprotein.PLoS pathogens · 2010Article
- Constitutive non-canonical NFkappaB signaling in pancreatic cancer cells.Cancer biology & therapy · 2009Article
- Adoptive immunotherapy: good habits instilled at youth have long-term benefits.Immunologic research · 2008Review
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Authors and funding
8 authors.
Funding
Abstract
To increase the efficiency of stable cell line establishment from primary ovarian cancer specimens, we simultaneously initiated cultures under multiple conditions, varying extracellular matrices and the inclusion of supplements (e.g., serum or serum albumin), while minimizing exposure to xenogeneic antigens (e.g., fetal calf serum). Primary cultures were initiated from 30 specimens; cell lines were established from 10 of these for a success rate of 33%. In some instances, multiple cell lines were established from the same specimen. Five lines were characterized extensively with respect to growth properties, antigen expression, and genomic alterations. Although these lines are all low-passage, marked heterogeneity was observed, even between lines derived from the same specimen. The culture approach outlined herein will facilitate generation of reagents useful for many aspects of ovarian cancer biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.