ArticleMolecular genetics and genomics : MGG2006
Functional and physical interactions within the middle domain of the yeast mediator.
Article in Molecular genetics and genomics : MGG, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- PsAvh109 suppresses SA-triggered immunity by mimicking TPL function to disrupt mediator complex assembly.Nature communications · 2026Article
- Repression by theeLife · 2021Article
- Interaction map of Arabidopsis Mediator complex expounding its topology.Nucleic acids research · 2019Article
- Article
- Tomato histone H2B monoubiquitination enzymes SlHUB1 and SlHUB2 contribute to disease resistance against Botrytis cinerea through modulating the balance between SA- and JA/ET-mediated signaling pathways.BMC plant biology · 2015Article
- Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.Cell · 2014Article
- Repressive chromatin affects factor binding at yeast HO (homothallic switching) promoter.The Journal of biological chemistry · 2011Article
- Gene-specific transcription activation via long-range allosteric shape-shifting.The Biochemical journal · 2011Review
- HISTONE MONOUBIQUITINATION1 interacts with a subunit of the mediator complex and regulates defense against necrotrophic fungal pathogens in Arabidopsis.The Plant cell · 2009Article
- Spreading of a corepressor linked to action of long-range repressor hairy.Molecular and cellular biology · 2008Article
- The Mediator complex and transcription regulation.Critical reviews in biochemistry and molecular biologyReview
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Med21 (Srb7) is a small essential subunit of the middle domain of the Mediator, which is conserved in all eukaryotes. It is thought to play an important role in both transcriptional activation and repression. In the yeast Saccharomyces cerevisiae, Med21 is known to interact both with the Mediator subunit Med6 and the global co-repressor Tup1. We have made a temperature-sensitive med21-ts mutant, which we used in a high copy number suppressor screen. We found ten yeast genes that can suppress the med21-ts mutation in high copy number. The three strongest suppressors were MED7 and MED10 (NUT2), which encode other Mediator subunits, and ASH1, which encodes a repressor of the HO gene. 2-Hybrid experiments confirmed multiple interactions between Med21, Med10, Med7 and Med4, and also revealed a Med21 self-interaction. The interactions of Med21 with Med7 and Med10 were verified by co-immunoprecipitation of tagged proteins produced in insect cells and E. coli, where both interactions were found to depend strongly on the amino acid residues 2-8 of Med21. These interactions, and the interactions of Med21 with Med6 and Tup1, suggest that Med21 may serve as a molecular switchboard that integrates different signals before they reach the core polymerase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.