ArticleEukaryotic cell2006
Lipid raft-based membrane compartmentation of a plant transport protein expressed in Saccharomyces cerevisiae.
Article in Eukaryotic cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 63 citations in OpenAlex.
- The distribution of phosphatidylinositol 4,5-bisphosphate in the budding yeast plasma membrane.Histochemistry and cell biology · 2021Article
- Article
- Role of MCC/Eisosome in Fungal Lipid Homeostasis.Biomolecules · 2019Review
- Integration Through Separation - The Role of Lateral Membrane Segregation in Nutrient Uptake.Frontiers in cell and developmental biology · 2019Review
- The Dynamics of Plasma Membrane, Metabolism and Respiration (PM-M-R) inFrontiers in microbiology · 2017Article
- Phosphatidylserine translocation at the yeast trans-Golgi network regulates protein sorting into exocytic vesicles.Molecular biology of the cell · 2015Article
- Characterization of three ammonium transporters of the glomeromycotan fungus Geosiphon pyriformis.Eukaryotic cell · 2013Article
- Seg1 controls eisosome assembly and shape.The Journal of cell biology · 2012Article
- Cell wall constrains lateral diffusion of plant plasma-membrane proteins.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Proteomic analysis of Rta2p-dependent raft-association of detergent-resistant membranes in Candida albicans.PloS one · 2012Article
- A genomewide screen for tolerance to cationic drugs reveals genes important for potassium homeostasis in Saccharomyces cerevisiae.Eukaryotic cell · 2011Article
- Lipid raft localization of EGFR alters the response of cancer cells to the EGFR tyrosine kinase inhibitor gefitinib.Journal of cellular physiology · 2011Article
- Mathematical modeling and validation of the ergosterol pathway in Saccharomyces cerevisiae.PloS one · 2011Article
- General and molecular microbiology and microbial genetics in the IM CAS.Journal of industrial microbiology & biotechnology · 2010Review
- Opportunities to explore plant membrane organization with super-resolution microscopy.Plant physiology · 2010Article
- Article
- Remorin, a solanaceae protein resident in membrane rafts and plasmodesmata, impairs potato virus X movement.The Plant cell · 2009Article
- TORC2 plasma membrane localization is essential for cell viability and restricted to a distinct domain.Molecular biology of the cell · 2009Article
- Very long chain fatty acid and lipid signaling in the response of plants to pathogens.Plant signaling & behavior · 2009Review
- Plasma membrane microdomains regulate turnover of transport proteins in yeast.The Journal of cell biology · 2008Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hexose-proton symporter HUP1 shows a spotty distribution in the plasma membrane of the green alga Chlorella kessleri. Chlorella cannot be transformed so far. To study the membrane localization of the HUP1 protein in detail, the symporter was fused to green fluorescent protein (GFP) and heterologously expressed in Saccharomyces cerevisiae and Schizosaccharomyces pombe. In these organisms, the HUP1 protein has previously been shown to be fully active. The GFP fusion protein was exclusively targeted to the plasma membranes of both types of fungal cells. In S. cerevisiae, it was distributed nonhomogenously and concentrated in spots resembling the patchy appearance observed previously for endogenous H(+) symporters. It is documented that the Chlorella protein colocalizes with yeast proteins that are concentrated in 300-nm raft-based membrane compartments. On the other hand, it is completely excluded from the raft compartment housing the yeast H(+)/ATPase. As judged by their solubilities in Triton X-100, the HUP1 protein extracted from Chlorella and the GFP fusion protein extracted from S. cerevisiae are detergent-resistant raft proteins. S. cerevisiae mutants lacking the typical raft lipids ergosterol and sphingolipids showed a homogenous distribution of HUP1-GFP within the plasma membrane. In an ergosterol synthesis (erg6) mutant, the rate of glucose uptake was reduced to less than one-third that of corresponding wild-type cells. In S. pombe, the sterol-rich plasma membrane domains can be stained in vivo with filipin. Chlorella HUP1-GFP accumulated exactly in these domains. Altogether, it is demonstrated here that a plant membrane protein has the property of being concentrated in specific raft-based membrane compartments and that the information for its raft association is retained between even distantly related organisms.
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