ArticleEukaryotic cell2006
Identification of the major spliceosomal RNAs in Dictyostelium discoideum reveals developmentally regulated U2 variants and polyadenylated snRNAs.
Article in Eukaryotic cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 41 citations in OpenAlex.
- Comparative genomics of two protozoansHeliyon · 2024Article
- Chromosome-level genome assembly and annotation of the social amoeba Dictyostelium firmibasis.Scientific data · 2024Article
- Abundantly expressed class of noncoding RNAs conserved through the multicellular evolution of dictyostelid social amoebas.Genome research · 2021Article
- Rapid protein evolution, organellar reductions, and invasive intronic elements in the marine aerobic parasite dinoflagellate Amoebophrya spp.BMC biology · 2021Article
- Nucleomorph Small RNAs in Cryptophyte and Chlorarachniophyte Algae.Genome biology and evolution · 2019Article
- Spliceosomal introns in Trichomonas vaginalis revisited.Parasites & vectors · 2018Article
- CRISPR/Cas9 mediated targeting of multiple genes in Dictyostelium.Scientific reports · 2018Article
- Global characterization of the Dicer-like protein DrnB roles in miRNA biogenesis in the social amoeba Dictyostelium discoideum.RNA biology · 2018Article
- The Long Noncoding RNA Transcriptome ofG3 (Bethesda, Md.) · 2017Article
- Variant U1 snRNAs are implicated in human pluripotent stem cell maintenance and neuromuscular disease.Nucleic acids research · 2016Article
- The C-Terminal SynMuv/DdDUF926 Domain Regulates the Function of the N-Terminal Domain of DdNKAP.PloS one · 2016Article
- Developmental analysis of spliceosomal snRNA isoform expression.G3 (Bethesda, Md.) · 2014Article
- Article
- A phylogenetic study ofOpen journal of animal sciences · 2013Article
- A rare combination of ribonucleotide reductases in the social amoeba Dictyostelium discoideum.The Journal of biological chemistry · 2013Article
- MicroRNAs in Amoebozoa: deep sequencing of the small RNA population in the social amoeba Dictyostelium discoideum reveals developmentally regulated microRNAs.RNA (New York, N.Y.) · 2012Article
- Sequence and generation of mature ribosomal RNA transcripts in Dictyostelium discoideum.The Journal of biological chemistry · 2011Article
- Spliceosomal genes in the D. discoideum genome: a comparison with those in H. sapiens, D. melanogaster, A. thaliana and S. cerevisiae.Protein & cell · 2011Article
- Comparative genomics of the social amoebae Dictyostelium discoideum and Dictyostelium purpureum.Genome biology · 2011Article
- Evolution of spliceosomal snRNA genes in metazoan animals.Journal of molecular evolution · 2008Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most eukaryotic mRNAs depend upon precise removal of introns by the spliceosome, a complex of RNAs and proteins. Splicing of pre-mRNA is known to take place in Dictyostelium discoideum, and we previously isolated the U2 spliceosomal RNA experimentally. In this study, we identified the remaining major spliceosomal RNAs in Dictyostelium by a bioinformatical approach. Expression was verified from 17 small nuclear RNA (snRNA) genes. All these genes are preceded by a putative noncoding RNA gene promoter. Immunoprecipitation showed that snRNAs U1, U2, U4, and U5, but not U6, carry the conserved trimethylated 5' cap structure. A number of divergent U2 species are expressed in Dictyostelium. These RNAs carry the U2 RNA hallmark sequence and structure motifs but have an additional predicted stem-loop structure at the 5' end. Surprisingly, and in contrast to the other spliceosomal RNAs in this study, the new U2 variants were enriched in the cytoplasm and were developmentally regulated. Furthermore, all of the snRNAs could also be detected as polyadenylated species, and polyadenylated U1 RNA was demonstrated to be located in the cytoplasm.
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