ArticleJournal of clinical microbiology2006
Combining multiplex reverse transcription-PCR and a diagnostic microarray to detect and differentiate enterovirus 71 and coxsackievirus A16.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 62 citations in OpenAlex.
- Trial
- Identification of enterovirus 71 hand, foot and mouth disease from other enteroviruses by microfluidic digital PCR.BMC infectious diseases · 2025Article
- Role of Non-Coding RNA in Neurological Complications Associated With Enterovirus 71.Frontiers in cellular and infection microbiology · 2022Review
- The association between diurnal temperature range and childhood hand, foot, and mouth disease: a distributed lag non-linear analysis.Epidemiology and infection · 2017Article
- Development of a highly sensitive real-time nested RT-PCR assay in a single closed tube for detection of enterovirus 71 in hand, foot, and mouth disease.Archives of virology · 2016Article
- The Association between Ambient Temperature and Childhood Hand, Foot, and Mouth Disease in Chengdu, China: A Distributed Lag Non-linear Analysis.Scientific reports · 2016Article
- Article
- Coxsackievirus A16: epidemiology, diagnosis, and vaccine.Human vaccines & immunotherapeutics · 2014Review
- Article
- An integrated lab-on-chip for rapid identification and simultaneous differentiation of tropical pathogens.PLoS neglected tropical diseases · 2014Article
- Expression of enterovirus 71 capsid protein VP1 in Escherichia coli and its clinical application.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2013Article
- The association of recombination events in the founding and emergence of subgenogroup evolutionary lineages of human enterovirus 71.Journal of virology · 2012Article
- Simultaneously typing nine serotypes of enteroviruses associated with hand, foot, and mouth disease by a GeXP analyzer-based multiplex reverse transcription-PCR assay.Journal of clinical microbiology · 2012Article
- The association between enterovirus 71 infections and meteorological parameters in Taiwan.PloS one · 2012Article
- External quality assessment for enterovirus 71 and coxsackievirus A16 detection by reverse transcription-PCR using armored RNA as a virus surrogate.Journal of clinical microbiology · 2011Article
- Development and evaluation of reverse transcription-loop-mediated isothermal amplification assay for rapid detection of enterovirus 71.BMC infectious diseases · 2011Article
- Molecular identification and analysis of nonserotypeable human enteroviruses.Journal of clinical microbiology · 2010Article
- Identification of 20 common human enterovirus serotypes by use of a reverse transcription-PCR-based reverse line blot hybridization assay.Journal of clinical microbiology · 2009Article
- Typing of enteroviruses by use of microwell oligonucleotide arrays.Journal of clinical microbiology · 2009Article
- New tools for the study and direct surveillance of viral pathogens in water.Current opinion in biotechnology · 2008Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cluster A enteroviruses, including enterovirus 71 (EV71) and coxsackievirus A16 (CA16), are known to cause hand-foot-and-mouth disease (HFMD). Despite the close genetic relationship between these two viruses, EV71 is generally known to be a more perpetuating pathogen involved in severe clinical manifestations and deaths. While the serotyping of enteroviruses is mostly done by conventional immunological methods, many clinical isolates remain unclassifiable due to the limited number of antibodies against enterovirus surface proteins. Array-based assays are able to detect several serotypes with high accuracy. We combined an enterovirus microarray with multiplex reverse transcription-PCR to try to develop a method of sensitively and accurately detecting and differentiating EV71 and CA16. In an effort to design serotype-specific probes for detection of the virus, we first did an elaborate bioinformatic analysis of the sequence database derived from different enterovirus serotypes. We then constructed a microarray using 60-mer degenerate oligonucleotide probes covalently bound to array slides. Using this enterovirus microarray to study 144 clinical specimens from patients infected with HFMD or suspected to have HFMD, we found that it had a diagnostic accuracy of 92.0% for EV71 and 95.8% for CA16. Diagnostic accuracy for other enteroviruses (non-EV71 or -CA16) was 92.0%. All specimens were analyzed in parallel by real-time PCR and subsequently confirmed by neutralization tests. This highly sensitive array-based assay may become a useful alternative in clinical diagnostics of EV71 and CA16.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.