ArticleNucleic acids research2006
Purification and preconcentration of genomic DNA from whole cell lysates using photoactivated polycarbonate (PPC) microfluidic chips.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 90 citations in OpenAlex.
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- A 3D-Printed Millifluidic Platform Enabling Bacterial Preconcentration and DNA Purification for Molecular Detection of Pathogens in Blood.Micromachines · 2018Article
- Simple Approaches to Minimally-Instrumented, Microfluidic-Based Point-of-Care Nucleic Acid Amplification Tests.Biosensors · 2018Review
- Most probable number - loop mediated isothermal amplification (MPN-LAMP) for quantifying waterborne pathogens in <25min.Journal of microbiological methods · 2017Article
- Simultaneously sizing and quantitating zeptomole-level DNA at high throughput in free solution.Chemistry (Weinheim an der Bergstrasse, Germany) · 2014Article
- Solid-phase extraction and purification of membrane proteins using a UV-modified PMMA microfluidic bioaffinity μSPE device.The Analyst · 2014Article
- Rapid extraction and preservation of genomic DNA from human samples.Analytical and bioanalytical chemistry · 2013Article
- Modular microfluidic system fabricated in thermoplastics for the strain-specific detection of bacterial pathogens.Lab on a chip · 2012Article
- Fully integrated thermoplastic genosensor for the highly sensitive detection and identification of multi-drug-resistant tuberculosis.Angewandte Chemie (International ed. in English) · 2012Article
- Design and development of a field-deployable single-molecule detector (SMD) for the analysis of molecular markers.The Analyst · 2012Article
- Flexible fabrication and applications of polymer nanochannels and nanoslits.Chemical Society reviews · 2011Review
- Perspective on optical biosensors and integrated sensor systems.Analytical chemistry · 2009Review
- 96-well polycarbonate-based microfluidic titer plate for high-throughput purification of DNA and RNA.Analytical chemistry · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We discuss the use of a photoactivated polycarbonate (PPC) microfluidic chip for the solid-phase, reversible immobilization (SPRI) and purification of genomic DNA (gDNA) from whole cell lysates. The surface of polycarbonate was activated by UV radiation resulting in a photo-oxidation reaction, which produced a channel surface containing carboxylate groups. The gDNA was selectively captured on this photoactivated surface in an immobilization buffer, which consisted of 3% polyethylene glycol, 0.4 M NaCl and 70% ethanol. The methodology reported herein is similar to conventional SPRI in that surface-confined carboxylate groups are used for the selective immobilization of DNA; however, no magnetic beads or a magnetic field are required. As observed by UV spectroscopy, a load of approximately 7.6 +/- 1.6 microg/ml of gDNA was immobilized onto the PPC bed. The recovery of DNA following purification was estimated to be 85 +/- 5%. The immobilization and purification assay using this PPC microchip could be performed within approximately 25 min as follows: (i) DNA immobilization approximately 6 min, (ii) chip washout with ethanol 10 min, and (iii) drying and gDNA desorption approximately 6 min. The PPC microchip could also be used for subsequent assays with no substantial loss in recovery, no observable carryover and no need for 'reactivation' of the PC surface with UV light.
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