ArticleVirology2006
A cis-replication element functions in both orientations to enhance replication of Turnip crinkle virus.
Article in Virology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Structural Analysis and Whole Genome Mapping of a New Type of Plant Virus Subviral RNA: Umbravirus-Like Associated RNAs.Viruses · 2021Article
- Development of a Viral RdRp-Assisted Gene Silencing System and Its Application in the Identification of Host Factors of Plant (+)RNA Virus.Frontiers in microbiology · 2021Article
- Putative Auxin and Light Responsive Promoter Elements From theFrontiers in plant science · 2019Article
- Modification of betanodavirus virulence by substitutions in the 3' terminal region of RNA2.The Journal of general virology · 2018Article
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- The Functional Roles of theFrontiers in microbiology · 2017Review
- Rapid evolution of in vivo-selected sequences and structures replacing 20% of a subviral RNA.Virology · 2015Article
- Evidence of pervasive biologically functional secondary structures within the genomes of eukaryotic single-stranded DNA viruses.Journal of virology · 2014Article
- Maintaining the structural integrity of the Bamboo mosaic virus 3' untranslated region is necessary for retaining the catalytic constant for minus-strand RNA synthesis.Virology journal · 2013Article
- A local, interactive network of 3' RNA elements supports translation and replication of Turnip crinkle virus.Journal of virology · 2012Article
- Hsp90 interacts specifically with viral RNA and differentially regulates replication initiation of Bamboo mosaic virus and associated satellite RNA.PLoS pathogens · 2012Article
- Multifaceted regulation of translational readthrough by RNA replication elements in a tombusvirus.PLoS pathogens · 2011Article
- Evolution of a helper virus-derived, ribosome binding translational enhancer in an untranslated satellite RNA of Turnip crinkle virus.Virology · 2011Article
- Ribosome binding to a 5' translational enhancer is altered in the presence of the 3' untranslated region in cap-independent translation of turnip crinkle virus.Journal of virology · 2011Article
- Article
- Satellite RNAs and Satellite Viruses of Plants.Viruses · 2009Article
- The 3' end of Turnip crinkle virus contains a highly interactive structure including a translational enhancer that is disrupted by binding to the RNA-dependent RNA polymerase.RNA (New York, N.Y.) · 2009Article
- Structural plasticity and rapid evolution in a viral RNA revealed by in vivo genetic selection.Journal of virology · 2009Article
- The 3' proximal translational enhancer of Turnip crinkle virus binds to 60S ribosomal subunits.RNA (New York, N.Y.) · 2008Article
- Structural domains within the 3' untranslated region of Turnip crinkle virus.Journal of virology · 2008Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Turnip crinkle virus (TCV) (family Tombusviridae, genus Carmovirus) is a positive-sense RNA virus containing a 4054-base genome. Previous results indicated that insertion of Hairpin 4 (H4) into a TCV-associated satellite RNA enhanced replication 6-fold in vivo (Nagy, P., Pogany, J., Simon, A. E., 1999. EMBO J. 18:5653-5665). A detailed structural and functional analysis of H4 has now been performed to investigate its role in TCV replication. RNA structural probing of H4 in full-length TCV supported the sequence forming hairpin structures in both orientations in vitro. Deletion and mutational analyses determined that H4 is important for efficient accumulation of TCV in protoplasts, with a 98% reduction of genomic RNA levels when H4 was deleted. In vitro transcription using p88 [the TCV RNA-dependent RNA polymerase] demonstrated that H4 in its plus-sense orientation [H4(+)] caused a nearly 2-fold increase in RNA synthesis from a core hairpin promoter located on TCV plus-strands. H4 in its minus-sense orientation [H4(-)] stimulated RNA synthesis by 100-fold from a linear minus-strand promoter. Gel mobility shift assays indicated that p88 binds H4(+) and H4(-) with equal affinity, which was substantially greater than the binding affinity to the core promoters. These results support roles for H4(+) and H4(-) in TCV replication by enhancing syntheses of both strands through attracting the RdRp to the template.
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Registered trials
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