ArticleProceedings of the National Academy of Sciences of the United States of America2006
Dimeric organization of the yeast oligosaccharyl transferase complex.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- Uncoupling the hydrolysis of lipid-linked oligosaccharide from the oligosaccharyl transfer reaction by point mutations in yeast oligosaccharyltransferase.The Journal of biological chemistry · 2020Article
- Comparative and evolutionary analyses of the divergence of plant oligosaccharyltransferase STT3 isoforms.FEBS open bio · 2020Article
- Glycosyltransferase complexes in eukaryotes: long-known, prevalent but still unrecognized.Cellular and molecular life sciences : CMLS · 2016Review
- Generation and degradation of free asparagine-linked glycans.Cellular and molecular life sciences : CMLS · 2015Review
- Eukaryotic oligosaccharyltransferase generates free oligosaccharides during N-glycosylation.The Journal of biological chemistry · 2013Article
- OST4 is a subunit of the mammalian oligosaccharyltransferase required for efficient N-glycosylation.Journal of cell science · 2013Article
- Review
- Biochemical characterization of cone cyclic nucleotide-gated (CNG) channel using the infrared fluorescence detection system.Advances in experimental medicine and biology · 2012Article
- Synthesis and secretion of gonadotropins including structure-function correlates.Reviews in endocrine & metabolic disorders · 2011Review
- DC2 and keratinocyte-associated protein 2 (KCP2), subunits of the oligosaccharyltransferase complex, are regulators of the gamma-secretase-directed processing of amyloid precursor protein (APP).The Journal of biological chemistry · 2011Article
- Comparative structural biology of eubacterial and archaeal oligosaccharyltransferases.The Journal of biological chemistry · 2010Article
- Oligosaccharyltransferase directly binds to ribosome at a location near the translocon-binding site.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Chemical cross-linking provides a model of the gamma-secretase complex subunit architecture and evidence for close proximity of the C-terminal fragment of presenilin with APH-1.The Journal of biological chemistry · 2008Article
- Molecular dynamics simulation of Kv channel voltage sensor helix in a lipid membrane with applied electric field.Biophysical journal · 2008Article
- Ribophorin I regulates substrate delivery to the oligosaccharyltransferase core.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Structure-guided identification of a new catalytic motif of oligosaccharyltransferase.The EMBO journal · 2008Article
- Dolichol-linked oligosaccharide selection by the oligosaccharyltransferase in protist and fungal organisms.The Journal of cell biology · 2007Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The enzyme complex oligosaccharyl transferase (OT) catalyzes N-glycosylation in the lumen of the endoplasmic reticulum. The yeast OT complex is composed of nine subunits, all of which are transmembrane proteins. Several lines of evidence, including our previous split-ubiquitin studies, have suggested an oligomeric organization of the OT complex, but the exact oligomeric nature has been unclear. By FLAG epitope tagging the Ost4p subunit of the OT complex, we purified the OT enzyme complex by using the nondenaturing detergent digitonin and a one-step immunoaffinity technique. The digitonin-solubilized OT complex was catalytically active, and all nine subunits were present in the enzyme complex. The purified OT complex had an apparent mass of approximately 500 kDa, suggesting a dimeric configuration, which was confirmed by biochemical studies. EM showed homogenous individual particles and revealed a dimeric structure of the OT complexes that was consistent with our biochemical studies. A 3D structure of the dimeric OT complex at 25-A resolution was reconstructed from EM images. We suggest that the dimeric structure of OT might be required for effective association with the translocon dimer and for its allosteric regulation during cotranslational glycosylation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.