Evidence map›Paper›PMID 16752082›Full record

ArticleMolecular and cellular biochemistry2006

Rab5b localization to early endosomes in the protozoan human pathogen Leishmania donovani.

Diane E Marotta, Noel Gerald, Dennis M Dwyer

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In one paragraph

Article in Molecular and cellular biochemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Diane E MarottaCell Biology Section, Laboratory of Parasitic Diseases, NIAID, National Institutes of Health, Bethesda, Maryland 20892-0425, USA. marottad@mail.med.upenn.edu
Noel Gerald
Dennis M Dwyer
National Institutes of Health · USCalifornia University of Pennsylvania · US

Funding

Biochemical And Molecular Characterization Of Enzymes Secreted By LeishmaniaZIAAI000745 · NIAID · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · PI DWYER, DENNIS · 2009 to 2012
$1.2M
Cell And Developmental Biology: Trypanosomatid ParasitesZ01AI000162 · NIAID · NIAID EXTRAMURAL ACTIVITIES · PI DWYER, DENNIS · 1985 to 2008
$415k
BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF ENZYMES SECRETED BY LEISHMANIAZ01AI000745 · NIAID · NIAID EXTRAMURAL ACTIVITIES · PI DWYER, DENNIS · 1996 to 2008
$335k
Intramural NIH HHS
6 · The paper itself

Abstract

Leishmania donovani is a primitive trypanosomatid pathogen of humans. This protozoan is apically polarized such that the flagellar reservoir, the exclusive site of endocytosis and exocytosis, is situated at the anterior end. Recent evidence for the existence of an endocytic pathway in Leishmania has prompted us to investigate candidate temporal markers for endocytosis. In this study we identify the L. donovani Rab5b gene, and demonstrate the localization of a Rab5b chimera to early endosomes. A full-length Rab5b protein was fused to green fluorescent protein (GFP) to generate a chimeric protein GFP::Rab5b. Transfected L. donovani promastigotes carrying this chimeric construct displayed GFP::Rab5b localization. Additionally, incubation of transfected promastigotes with the fluid-phase marker Texas Red dextran demonstrated anterior co-localization of GFP::Rab5b and dye. This suggests Rab5b may act as a marker for early endosomes in L. donovani.

Indexed as

Amino Acid SequenceAnimalsDextransEndosomesGene Expression RegulationGreen Fluorescent ProteinsHumansLeishmania donovaniMolecular Sequence DataPhylogenyPlasmidsProtein TransportProtozoan Proteinsrab5 GTP-Binding ProteinsRecombinant Fusion ProteinsRNA, MessengerDextransGreen Fluorescent ProteinsProtozoan Proteinsrab5 GTP-Binding ProteinsRecombinant Fusion ProteinsRNA, MessengerTexas redXanthenes

Identifiers

PMID16752082
OpenAlexW2018952567

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.