ArticleApplied and environmental microbiology2006
Construction of a large signature-tagged mini-Tn5 transposon library and its application to mutagenesis of Sinorhizobium meliloti.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Recent Advances in Genetic Engineering Strategies ofACS synthetic biology · 2024Review
- Tyrosine Nitration of Flagellins: a Response of Sinorhizobium meliloti to Nitrosative Stress.Applied and environmental microbiology · 2020Article
- The Endoribonuclease RNase E Coordinates Expression of mRNAs and Small Regulatory RNAs and Is Critical for the Virulence of Brucella abortus.Journal of bacteriology · 2020Article
- Identification ofFrontiers in microbiology · 2019Article
- The Nitrate Assimilatory Pathway inFrontiers in microbiology · 2019Article
- Novel Genes and Regulators That Influence Production of Cell Surface Exopolysaccharides in Sinorhizobium meliloti.Journal of bacteriology · 2018Article
- An sRNA and Cold Shock Protein Homolog-Based Feedforward Loop Post-transcriptionally Controls Cell Cycle Master Regulator CtrA.Frontiers in microbiology · 2018Article
- Article
- Anoxic growth of Ensifer meliloti 1021 by N2O-reduction, a potential mitigation strategy.Frontiers in microbiology · 2015Article
- Genetic basis for denitrification in Ensifer meliloti.BMC microbiology · 2014Article
- RNase E affects the expression of the acyl-homoserine lactone synthase gene sinI in Sinorhizobium meliloti.Journal of bacteriology · 2014Article
- Riboregulation in plant-associated α-proteobacteria.RNA biology · 2014Review
- The DivJ, CbrA and PleC system controls DivK phosphorylation and symbiosis in Sinorhizobium meliloti.Molecular microbiology · 2013Article
- Inability to catabolize galactose leads to increased ability to compete for nodule occupancy in Sinorhizobium meliloti.Journal of bacteriology · 2012Article
- A comparative genomics screen identifies a Sinorhizobium meliloti 1021 sodM-like gene strongly expressed within host plant nodules.BMC microbiology · 2012Article
- ExpR is not required for swarming but promotes sliding in Sinorhizobium meliloti.Journal of bacteriology · 2012Article
- Sinorhizobium meliloti sigma factors RpoE1 and RpoE4 are activated in stationary phase in response to sulfite.PloS one · 2012Article
- The RpiR-like repressor IolR regulates inositol catabolism in Sinorhizobium meliloti.Journal of bacteriology · 2011Article
- Both plant and bacterial nitrate reductases contribute to nitric oxide production in Medicago truncatula nitrogen-fixing nodules.Plant physiology · 2011Article
- Silent mischief: bacteriophage Mu insertions contaminate products of Escherichia coli random mutagenesis performed using suicidal transposon delivery plasmids mobilized by broad-host-range RP4 conjugative machinery.Journal of bacteriology · 2010Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sinorhizobium meliloti genome sequence determination has provided the basis for different approaches of functional genomics for this symbiotic nitrogen-fixing alpha-proteobacterium. One of these approaches is gene disruption with subsequent analysis of mutant phenotypes. This method is efficient for single genes; however, it is laborious and time-consuming if it is used on a large scale. Here, we used a signature-tagged transposon mutagenesis method that allowed analysis of the survival and competitiveness of many mutants in a single experiment. A novel set of signature tags characterized by similar melting temperatures and G+C contents of the tag sequences was developed. The efficiencies of amplification of all tags were expected to be similar. Thus, no preselection of the tags was necessary to create a library of 412 signature-tagged transposons. To achieve high specificity of tag detection, each transposon was bar coded by two signature tags. In order to generate defined, nonredundant sets of signature-tagged S. meliloti mutants for subsequent experiments, 12,000 mutants were constructed, and insertion sites for more than 5,000 mutants were determined. One set consisting of 378 mutants was used in a validation experiment to identify mutants showing altered growth patterns.
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