ArticleApplied and environmental microbiology2006
Comparison of mechanisms of alkane metabolism under sulfate-reducing conditions among two bacterial isolates and a bacterial consortium.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 117 citations in OpenAlex.
- Advances in polyethylene biodegradation and bioconversion: Microbial, enzymatic, and biotechnological insights.Engineering microbiology · 2026Review
- Optimizing a carbon source based on water temperature to reduceFrontiers in systems biology · 2026Article
- Nitrate-driven anaerobic oxidation of ethane and butane by bacteria.The ISME journal · 2024Article
- Subsurface hydrocarbon degradation strategies in low- and high-sulfate coal seam communities identified with activity-based metagenomics.NPJ biofilms and microbiomes · 2022Article
- A review on microbial diversity and genetic markers involved in methanogenic degradation of hydrocarbons: futuristic prospects of biofuel recovery from contaminated regions.Environmental science and pollution research international · 2021Review
- Methanogenic Paraffin Biodegradation: Alkylsuccinate Synthase Gene Quantification and Dicarboxylic Acid Production.Applied and environmental microbiology · 2018Article
- Metagenomic Characterization of Candidatus Smithella cisternae Strain M82_1, a Syntrophic Alkane-Degrading Bacteria, Enriched from the Shengli Oil Field.Microbes and environments · 2017Article
- Anaerobic Oxidation of Ethane, Propane, and Butane by Marine Microbes: A Mini Review.Frontiers in microbiology · 2017Review
- Time Course-Dependent Methanogenic Crude Oil Biodegradation: Dynamics of Fumarate Addition Metabolites, Biodegradative Genes, and Microbial Community Composition.Frontiers in microbiology · 2017Article
- Structural Basis for Substrate Specificity in Adenosylcobalamin-dependent Isobutyryl-CoA Mutase and Related Acyl-CoA Mutases.The Journal of biological chemistry · 2015Article
- Insights into the Anaerobic Biodegradation Pathway of n-Alkanes in Oil Reservoirs by Detection of Signature Metabolites.Scientific reports · 2015Article
- The anaerobic degradation of gaseous, nonmethane alkanes - From in situ processes to microorganisms.Computational and structural biotechnology journal · 2015Review
- Ubiquitous Presence and Novel Diversity of Anaerobic Alkane Degraders in Cold Marine Sediments.Frontiers in microbiology · 2015Article
- High Diversity of Anaerobic Alkane-Degrading Microbial Communities in Marine Seep Sediments Based on (1-methylalkyl)succinate Synthase Genes.Frontiers in microbiology · 2015Article
- Anaerobic activation of p-cymene in denitrifying betaproteobacteria: methyl group hydroxylation versus addition to fumarate.Applied and environmental microbiology · 2014Article
- Anaerobic oxidation of long-chain n-alkanes by the hyperthermophilic sulfate-reducing archaeon, Archaeoglobus fulgidus.The ISME journal · 2014Article
- Conversion of crude oil to methane by a microbial consortium enriched from oil reservoir production waters.Frontiers in microbiology · 2014Article
- In situ detection of anaerobic alkane metabolites in subsurface environments.Frontiers in microbiology · 2013Article
- Enzymes involved in the anaerobic oxidation of n-alkanes: from methane to long-chain paraffins.Frontiers in microbiology · 2013Article
- Structural insights into diversity and n-alkane biodegradation mechanisms of alkane hydroxylases.Frontiers in microbiology · 2013Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent studies have demonstrated that fumarate addition and carboxylation are two possible mechanisms of anaerobic alkane degradation. In the present study, we surveyed metabolites formed during growth on hexadecane by the sulfate-reducing isolates AK-01 and Hxd3 and by a mixed sulfate-reducing consortium. The cultures were incubated with either protonated or fully deuterated hexadecane; the sulfate-reducing consortium was also incubated with [1,2-13C2]hexadecane. All cultures were extracted, silylated, and analyzed by gas chromatography-mass spectrometry. We detected a suite of metabolites that support a fumarate addition mechanism for hexadecane degradation by AK-01, including methylpentadecylsuccinic acid, 4-methyloctadecanoic acid, 4-methyloctadec-2,3-enoic acid, 2-methylhexadecanoic acid, and tetradecanoic acid. By using d34-hexadecane, mass spectral evidence strongly supporting a carbon skeleton rearrangement of the first intermediate, methylpentadecylsuccinic acid, was demonstrated for AK-01. Evidence indicating hexadecane carboxylation was not found in AK-01 extracts but was observed in Hxd3 extracts. In the mixed sulfate-reducing culture, however, metabolites consistent with both fumarate addition and carboxylation mechanisms of hexadecane degradation were detected, which demonstrates that multiple alkane degradation pathways can occur simultaneously within distinct anaerobic communities. Collectively, these findings underscore that fumarate addition and carboxylation are important alkane degradation mechanisms that may be widespread among phylogenetically and/or physiologically distinct microorganisms.
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