ArticleApplied and environmental microbiology2006
Improved enumeration of lactic acid bacteria in mesophilic dairy starter cultures by using multiplex quantitative real-time PCR and flow cytometry-fluorescence in situ hybridization.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 71 citations in OpenAlex.
- Lactic acid bacteria as an eco-friendly approach in plant production: Current state and prospects.Folia microbiologica · 2024Review
- New Genetic Determinants for qPCR Identification and the Enumeration of Selected Lactic Acid Bacteria in Raw-Milk Cheese.Molecules (Basel, Switzerland) · 2024Article
- A multifaceted investigation of lactococcal strain diversity in undefined mesophilic starter cultures.Applied and environmental microbiology · 2024Article
- Review
- Cultureless enumeration of live bacteria in urinary tract infection by single-cell Raman spectroscopy.Frontiers in microbiology · 2023Article
- Potential of Flow Cytometric Approaches for Rapid Microbial Detection and Characterization in the Food Industry-A Review.Foods (Basel, Switzerland) · 2021Review
- Flow-FISH Using Nucleic Acid Mimic Probes for the Detection of Bacteria.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Hydrocarbon-Degrading Bacteria Found Tightly Associated with the 50-70 μm Cell-Size Population of Eukaryotic Phytoplankton in Surface Waters of a Northeast Atlantic Region.Microorganisms · 2020Article
- Molecular typing tools for identifying and characterizing lactic acid bacteria: a review.Food science and biotechnology · 2020Review
- An important step forward for the future development of an easy and fast procedure for identifying the most dangerous wine spoilage yeast, Dekkera bruxellensis, in wine environment.Microbial biotechnology · 2019Article
- High-throughput sequencing for the detection of the bacterial and fungal diversity in Mongolian naturally fermented cow's milk in Russia.BMC microbiology · 2015Article
- Evolution of microbiological analytical methods for dairy industry needs.Frontiers in microbiology · 2014Review
- Development of a flow-fluorescence in situ hybridization protocol for the analysis of microbial communities in anaerobic fermentation liquor.BMC microbiology · 2013Article
- Cytofluorometric detection of wine lactic acid bacteria: application of malolactic fermentation to the monitoring.Journal of industrial microbiology & biotechnology · 2013Article
- Biodiversity in Oscypek, a traditional Polish cheese, determined by culture-dependent and -independent approaches.Applied and environmental microbiology · 2012Article
- Locked nucleic acid flow cytometry-fluorescence in situ hybridization (LNA flow-FISH): a method for bacterial small RNA detection.Journal of visualized experiments : JoVE · 2012Article
- Locked nucleic acid and flow cytometry-fluorescence in situ hybridization for the detection of bacterial small noncoding RNAs.Applied and environmental microbiology · 2012Article
- Cell viability and functionality of probiotic bacteria in dairy products.Frontiers in microbiology · 2011Article
- Quantitative real-time PCR and fluorescence in situ hybridization approaches for enumerating Brevundimonas diminuta in drinking water.Journal of industrial microbiology & biotechnology · 2010Article
- Signalling with retinoids in the human lung: validation of new tools for the expression study of retinoid receptors.BMC cancer · 2009Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleic acid-based assays were developed to enumerate members of the three taxa Lactococcus lactis subsp. cremoris, L. lactis subsp. lactis, and Leuconostoc spp. in mesophilic starter cultures. To our knowledge the present is the first study to present a multiplex quantitative PCR (qPCR) strategy for the relative enumeration of bacteria. The multiplex qPCR strategy was designed to quantify the target DNA simultaneously relative to total bacterial DNA. The assay has a high discriminatory power and resolves concentration changes as low as 1.3-fold. The methodology was compared with flow cytometric fluorescence in situ hybridization (FLOW-FISH) and 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (X-Gal)-calcium citrate agar-based plate counting. For enumeration by FLOW-FISH, three new probes having the same specificity as the qPCR assay were designed and established. A combination with flow cytometry greatly reduced the time consumed compared to manual enumeration. Both qPCR and FLOW-FISH yielded similar community compositions for 10 complex starter cultures, with all detected subpopulations being highly significantly correlated (P < 0.001). Correlations between X-Gal-calcium citrate agar-based CFU and qPCR-derived counts were highly significant (P < 0.01 and P < 0.001, respectively) for the number of acidifiers versus L. lactis subsp. cremoris and for Leuconostoc spp. as quantified by the two techniques, respectively. This confirmed that most acidifiers in the studied PROBAT cultures are members of L. lactis subsp. cremoris. Quantitative real-time PCR and FLOW-FISH were found to be effective and accurate tools for the bacterial community analysis of complex starter cultures.
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