ArticleApplied and environmental microbiology2006
Rapid one-step quantitative reverse transcriptase PCR assay with competitive internal positive control for detection of enteroviruses in environmental samples.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed.
- Sequence-specific nanoparticle barcode strategy for multiplex human enterovirus typing.Nature communications · 2024Article
- Article
- Comparative assessment of Nanotrap and polyethylene glycol-based virus concentration in wastewater samples.FEMS microbes · 2024Article
- Article
- Nucleic acid sample preparation from whole blood in a paper microfluidic device using isotachophoresis.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2021Article
- Identifying Inorganic Turbidity in Water Samples as Potential Loss Factor During Nucleic Acid Extraction: Implications for Molecular Fecal Pollution Diagnostics and Source Tracking.Frontiers in microbiology · 2021Article
- Optimizing an eDNA protocol for estuarine environments: Balancing sensitivity, cost and time.PloS one · 2020Article
- Assessment of enteroviruses from sewage water and clinical samples during eradication phase of polio in North India.Virology journal · 2018Article
- Persistence of Viruses by qPCR Downstream of Three Effluent-Dominated Rivers in the Western United States.Food and environmental virology · 2018Article
- Review
- Coxsackievirus B4 as a Causative Agent of Diabetes Mellitus Type 1: Is There a Role of Inefficiently Treated Drinking Water and Sewage in Virus Spreading?Food and environmental virology · 2018Article
- Quantitative CrAssphage PCR Assays for Human Fecal Pollution Measurement.Environmental science & technology · 2017Article
- Mutational Disruption of cis-Acting Replication Element 2C in Coxsackievirus B3 Leads to 5'-Terminal Genomic Deletions.Journal of virology · 2015Article
- Genes Indicative of Zoonotic and Swine Pathogens Are Persistent in Stream Water and Sediment following a Swine Manure Spill.Applied and environmental microbiology · 2015Article
- Detection of enterovirus D68 in Canadian laboratories.Journal of clinical microbiology · 2015Article
- Norovirus and rotavirus survival in urine collected from a public ecological sanitation system in Ouagadougou, Burkina Faso.Food and environmental virology · 2015Article
- Organic substances interfere with reverse transcription-quantitative PCR-based virus detection in water samples.Applied and environmental microbiology · 2015Article
- Mechanisms of antiviral action of plant antimicrobials against murine norovirus.Applied and environmental microbiology · 2014Article
- Assessment of animal impacts on bacterial water quality in a South Carolina, USA tidal creek system.Environmental monitoring and assessment · 2013Article
- Predictive models for Escherichia coli concentrations at inland lake beaches and relationship of model variables to pathogen detection.Applied and environmental microbiology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human enteroviruses can serve as a more accurate indicator of human fecal contamination than conventional bacteriological fecal indicators. We describe here a quantitative reverse transcriptase PCR (qRT-PCR) assay specifically tailored to detect these viruses in environmental waters. The assay included a competitive internal positive control (CIPC) that allowed the inhibition of qRT-PCRs to be quantitatively assessed. Coamplification of the CIPC with enteroviral genetic material did not affect the sensitivity, specificity, or reproducibility of the enteroviral qRT-PCR assay. The assay is rapid (less than 5 h from sample to result), has a wide dynamic range (>3 logs), and is capable of detecting as few as 25 enteroviral genomes with an average amplification efficiency of 0.91. In samples with low or moderate inhibition, the delay in CIPC amplification was used to adjust enterovirus qRT-PCR concentrations to account for losses due to inhibition. Samples exhibiting significant inhibition were not corrected but instead diluted twofold and immediately assayed again. Using significantly inhibited samples, it was found that dilution relieved inhibition in 93% (25 of 27) of the samples. In addition, 15% (4 of 27) of these previously negative samples contained enteroviral genomes. The high-throughput format of the assay compared to conventional culture-based methods offers a fast, reliable, and specific method for detecting enteroviruses in environmental water samples. The ability of the assay to identify false negatives and provide improved quantitative assessments of enterovirus concentrations will facilitate the tracking of human fecal contamination and the assessment of potential public health risk due to enteroviruses in recreational and shellfish harvesting waters.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.