ArticleApplied and environmental microbiology2006
Direct quantitation and detection of salmonellae in biological samples without enrichment, using two-step filtration and real-time PCR.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Optimizing Lysis and Extraction Workflows for Enrichment-Free qPCR Detection ofPathogens (Basel, Switzerland) · 2026Article
- Digital PCR assay for the specific detection and estimation ofCurrent research in food science · 2024Article
- Prevalence and antibiotic resistance ofFrontiers in microbiology · 2023Article
- Current Methods for Extraction and Concentration of Foodborne Bacteria with Glycan-Coated Magnetic Nanoparticles: A Review.Biosensors · 2022Review
- Recent advances in magnetic nanoparticle-based microfluidic devices for the pretreatment of pathogenic bacteria.Biomedical engineering letters · 2021Review
- A Microbial Monitoring System Demonstrated on the International Space Station Provides a Successful Platform for Detection of Targeted Microorganisms.Life (Basel, Switzerland) · 2021Article
- Electrochemical Immuno- and Aptamer-Based Assays for Bacteria: Pros and Cons over Traditional Detection Schemes.Sensors (Basel, Switzerland) · 2020Review
- Research Progress of M13 Bacteriophage-Based Biosensors.Nanomaterials (Basel, Switzerland) · 2019Review
- Detection ofBiosensors · 2019Article
- Development of a filtration-based LAMP-LFA method as sensitive and rapid detection ofJournal of food science and technology · 2019Article
- Presence and Persistence ofFrontiers in public health · 2018Review
- Real-Time PCR Method for Detection of Salmonella spp. in Environmental Samples.Applied and environmental microbiology · 2017Article
- Improved sample preparation for direct quantitative detection of Escherichia coli O157 in soil using qPCR without pre-enrichment.Microbial biotechnology · 2017Article
- Detection of Salmonella enterica in Meat in Less than 5 Hours by a Low-Cost and Noncomplex Sample Preparation Method.Applied and environmental microbiology · 2017Article
- Screening and DetectingFrontiers in microbiology · 2017Article
- Detection of Salmonella spp. from chevon, mutton and its environment in retail meat shops in Anand city (Gujarat), India.Veterinary world · 2015Article
- Enumeration of salmonellae in table eggs, pasteurized egg products, and egg-containing dishes by using quantitative real-time PCR.Applied and environmental microbiology · 2014Article
- Development of a sensitive and specific qPCR assay in conjunction with propidium monoazide for enhanced detection of live Salmonella spp. in food.BMC microbiology · 2013Article
- Rapid sample processing for detection of food-borne pathogens via cross-flow microfiltration.Applied and environmental microbiology · 2013Article
- Rapid filtration separation-based sample preparation method for Bacillus spores in powdery and environmental matrices.Applied and environmental microbiology · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new two-step filtration protocol followed by a real-time PCR assay based on SYBR green I detection was developed to directly quantitate salmonellae in two types of biological samples: i.e., chicken rinse and spent irrigation water. Four prefiltration filters, one type of final filter, and six protocols for recovery of salmonellae from the final filter were evaluated to identify an effective filtration protocol. This method was then combined with a real-time PCR assay based on detection of the invA gene. The best results were obtained by subsequent filtration of 100 ml of chicken rinse or 100 ml of spent irrigation water through filters with pore diameters of >40 mum to remove large particles and of 0.22 microm to recover the Salmonella cells. After this, the Salmonella cells were removed from the filter by vortexing in 1 ml of physiological saline, and this sample was then subjected to real-time quantitative PCR. The whole procedure could be completed within 3 h from sampling to quantitation, and cell numbers as low as 7.5 x 10(2) CFU per 100-ml sample could be quantified. Below this limit, qualitative detection of concentrations as low as 2.2 CFU/100 ml sample was possible on occasion. This study has contributed to the development of a simple, rapid, and reliable method for quantitation of salmonellae in food without the need for sample enrichment or DNA extraction.
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