ArticleNeuropharmacology2006
Differential targeting and function of alpha2A and alpha2C adrenergic receptor subtypes in cultured sympathetic neurons.
Article in Neuropharmacology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
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- Cardiorespiratory fitness mediates cortisol and lactate responses to winter and summer marches.Journal of medical biochemistry · 2024Article
- Deficiency of NOD1 Improves the β-Adrenergic Modulation of CaFrontiers in physiology · 2018Article
- The amyloid precursor protein modulates αFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Stress Hormones Epinephrine and Corticosterone Selectively Modulate Herpes Simplex Virus 1 (HSV-1) and HSV-2 Productive Infections in Adult Sympathetic, but Not Sensory, Neurons.Journal of virology · 2017Article
- α2-Adrenergic Receptor and Isoflurane Modulation of Presynaptic Ca2+ Influx and Exocytosis in Hippocampal Neurons.Anesthesiology · 2016Article
- Effects of COX inhibition and LPS on formalin induced pain in the infant rat.Developmental neurobiology · 2015Article
- Common α2A and α2C adrenergic receptor polymorphisms do not affect plasma membrane trafficking.Naunyn-Schmiedeberg's archives of pharmacology · 2014Article
- REEP1 and REEP2 proteins are preferentially expressed in neuronal and neuronal-like exocytotic tissues.Brain research · 2014Article
- Cross-talk from β-adrenergic receptors modulates α2A-adrenergic receptor endocytosis in sympathetic neurons via protein kinase A and spinophilin.The Journal of biological chemistry · 2013Article
- Article
- Systematic and quantitative analysis of G protein-coupled receptor trafficking motifs.Methods in enzymology · 2013Article
- α2 adrenergic receptor dysregulation in depressive disorders: implications for the neurobiology of depression and antidepressant therapy.Neuroscience and biobehavioral reviews · 2012Review
- Intracellular α(2C)-adrenoceptors: storage depot, stunted development or signaling domain?Biochimica et biophysica acta · 2011Review
- Regulation of G-protein coupled receptor traffic by an evolutionary conserved hydrophobic signal.Traffic (Copenhagen, Denmark) · 2010Article
- Epitope-tagged receptor knock-in mice reveal that differential desensitization of alpha2-adrenergic responses is because of ligand-selective internalization.The Journal of biological chemistry · 2009Article
- GPCR monomers and oligomers: it takes all kinds.Trends in neurosciences · 2008Review
- Organization of beta-adrenoceptor signaling compartments by sympathetic innervation of cardiac myocytes.The Journal of cell biology · 2007Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Previous research suggested that alpha2A and alpha2C adrenergic receptor (AR) subtypes have overlapping but unique physiological roles in neuronal signaling; however, the basis for these dissimilarities is not completely known. To better understand the observed functional differences between these autoreceptors, we investigated targeting and signaling of endogenously expressed alpha2A and alpha2CARs in cultured sympathetic ganglion neurons (SGN). At Days 1 and 4, alpha2A and alpha2CARs could be readily detected in SGN from wild-type mice. By Day 8, alpha2A ARs were targeted to cell body, as well as axonal and dendritic sites, whereas alpha2C ARs were primarily localized to an intracellular vesicular pool within the cell body and proximal dendritic projections. Expression of synaptic vesicle marker protein SV2 did not differ at Day 8 nor co-localize with either subtype. By Day 16, however, alpha2C ARs had relocated to somatodendritic and axonal sites and, unlike alpha2A ARs, co-localized with SV2 at synaptic contact sites. Consistent with a functional role for alpha2 ARs, we also observed that dexmedetomidine stimulation of cultured SGN more efficiently inhibited depolarization-induced calcium entry into older, compared to younger, cultures. These results provide direct evidence of distinct developmental patterns of endogenous alpha2A and alpha2C AR targeting and function in a native cell system and that maturation of SGN in culture leads to alterations in neuronal properties required for proper targeting. More importantly, the co-localization at Day 16 of alpha2C ARs at sites of synaptic contact may partially explain the differential modulation of neurotransmitter release and responsiveness to action potential frequency observed between alpha2A and alpha2C ARs in SGN.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.