ArticleJournal of bacteriology2006
Role for tandem duplication and lon protease in AcrAB-TolC- dependent multiple antibiotic resistance (Mar) in an Escherichia coli mutant without mutations in marRAB or acrRAB.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 58 citations in OpenAlex.
- Nonantibiotic-driven evolution reveals rare but predictable routes to broad antibiotic resistance.mBio · 2026Article
- Article
- Article
- Single-Cell Microfluidics: A Primer for Microbiologists.The journal of physical chemistry. B · 2024Review
- The Effect of the Stringent Response and Oxidative Stress Response on Fitness Costs of De Novo Acquisition of Antibiotic Resistance.International journal of molecular sciences · 2024Article
- Analysis of the evolution of resistance to multiple antibiotics enables prediction of the Escherichia coli phenotype-based fitness landscape.PLoS biology · 2022Article
- Identification of Evolutionary Trajectories Associated with Antimicrobial Resistance Using Microfluidics.ACS infectious diseases · 2022Article
- Dynamic Boolean modelling reveals the influence of energy supply on bacterial efflux pump expression.Journal of the Royal Society, Interface · 2022Article
- Role of the SOS Response in the Generation of Antibiotic ResistanceAntimicrobial agents and chemotherapy · 2021Article
- Novel Chromosomal Mutations Responsible for Fosfomycin Resistance inFrontiers in microbiology · 2020Article
- Vaccines Against Antimicrobial Resistance.Frontiers in immunology · 2020Review
- Genome rearrangements in Escherichia coli during de novo acquisition of resistance to a single antibiotic or two antibiotics successively.BMC genomics · 2018Article
- Article
- Isolating Escherichia coli strains for recombinant protein production.Cellular and molecular life sciences : CMLS · 2017Review
- Tetracycline Antibiotics and Resistance.Cold Spring Harbor perspectives in medicine · 2016Review
- Prediction of antibiotic resistance by gene expression profiles.Nature communications · 2014Article
- Genomics of rapid adaptation to antibiotics: convergent evolution and scalable sequence amplification.Genome biology and evolution · 2014Article
- Involvement of MarR and YedS in carbapenem resistance in a clinical isolate of Escherichia coli from China.Antimicrobial agents and chemotherapy · 2013Article
- SoxS increases the expression of the zinc uptake system ZnuACB in an Escherichia coli murine pyelonephritis model.Journal of bacteriology · 2012Article
- Phenotypic landscape of a bacterial cell.Cell · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A spontaneous mutant (M113) of Escherichia coli AG100 with an unstable multiple antibiotic resistance (Mar) phenotype was isolated in the presence of tetracycline. Two mutations were found: an insertion in the promoter of lon (lon3::IS186) that occurred first and a subsequent large tandem duplication, dupIS186, bearing the genes acrAB and extending from the lon3::IS186 to another IS186 present 149 kb away from lon. The decreased amount of Lon protease increased the amount of MarA by stabilization of the basal quantities of MarA produced, which in turn increased the amount of multidrug effux pump AcrAB-TolC. However, in a mutant carrying only a lon mutation, the overproduced pump mediated little, if any, increased multidrug resistance, indicating that the Lon protease was required for the function of the pump. This requirement was only partial since resistance was mediated when amounts of AcrAB in a lon mutant were further increased by a second mutation. In M113, amplification of acrAB on the duplication led to increased amounts of AcrAB and multidrug resistance. Spontaneous gene duplication represents a new mechanism for mediating multidrug resistance in E. coli through AcrAB-TolC.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.