ArticleJournal of bacteriology2006
The orphan response regulator DigR is required for synthesis of extracellular matrix fibrils in Myxococcus xanthus.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- A novel insight into ComE-mediated activation of gene expression inMicrobiology spectrum · 2025Article
- Substrate stiffness modulates collective colony expansion of the social bacteriumAPL bioengineering · 2025Article
- Article
- Myxobacterial Genomics and Post-Genomics: A Review of Genome Biology, Genome Sequences and Related 'Omics Studies.Microorganisms · 2021Review
- Characterization of the Exopolysaccharide Biosynthesis Pathway in Myxococcus xanthus.Journal of bacteriology · 2020Article
- TheJournal of bacteriology · 2017Article
- A Minimal Threshold of c-di-GMP Is Essential for Fruiting Body Formation and Sporulation in Myxococcus xanthus.PLoS genetics · 2016Article
- Cyclic Di-GMP Regulates Type IV Pilus-Dependent Motility in Myxococcus xanthus.Journal of bacteriology · 2016Article
- Contact- and Protein Transfer-Dependent Stimulation of Assembly of the Gliding Motility Machinery in Myxococcus xanthus.PLoS genetics · 2015Article
- Nutrient-regulated proteolysis of MrpC halts expression of genes important for commitment to sporulation during Myxococcus xanthus development.Journal of bacteriology · 2014Article
- Outside-in assembly pathway of the type IV pilus system in Myxococcus xanthus.Journal of bacteriology · 2014Article
- MasABK proteins interact with proteins of the type IV pilin system to affect social motility of Myxococcus xanthus.PloS one · 2013Article
- The orphan histidine protein kinase SgmT is a c-di-GMP receptor and regulates composition of the extracellular matrix together with the orphan DNA binding response regulator DigR in Myxococcus xanthus.Molecular microbiology · 2012Article
- Spore formation in Myxococcus xanthus is tied to cytoskeleton functions and polysaccharide spore coat deposition.Molecular microbiology · 2012Article
- Global transcriptome analysis of spore formation in Myxococcus xanthus reveals a locus necessary for cell differentiation.BMC genomics · 2010Article
- The atypical hybrid histidine protein kinase RodK in Myxococcus xanthus: spatial proximity supersedes kinetic preference in phosphotransfer reactions.Journal of bacteriology · 2009Article
- EspA, an orphan hybrid histidine protein kinase, regulates the timing of expression of key developmental proteins of Myxococcus xanthus.Journal of bacteriology · 2008Article
- Bioinformatics and experimental analysis of proteins of two-component systems in Myxococcus xanthus.Journal of bacteriology · 2008Article
- Transcriptome analysis of Pseudomonas putida KT2440 harboring the completely sequenced IncP-7 plasmid pCAR1.Journal of bacteriology · 2007Article
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Myxococcus xanthus, two-component systems have crucial roles in regulating motility behavior and development. Here we describe an orphan response regulator, consisting of an N-terminal receiver domain and a C-terminal DNA binding domain, which is required for A and type IV pilus-dependent gliding motility. Genetic evidence suggests that phosphorylation of the conserved, phosphorylatable aspartate residue in the receiver domain is required for DigR activity. Consistent with the defect in type IV pilus-dependent motility, a digR mutant is slightly reduced in type IV pilus biosynthesis, and the composition of the extracellular matrix fibrils is abnormal, with an increased content of polysaccharides and decreased accumulation of the FibA metalloprotease. By using genome-wide transcriptional profiling, 118 genes were identified that are directly or indirectly regulated by DigR. These 118 genes include only 2, agmQ and cheY4, previously implicated in A and type IV pilus-dependent motility, respectively. In silico analyses showed that 36% of the differentially expressed genes are likely to encode exported proteins. Moreover, four genes encoding homologs of extracytoplasmic function (ECF) sigma factors, which typically control aspects of cell envelope homeostasis, are differentially expressed in a digR mutant. We suggest that the DigR response regulator has an important function in cell envelope homeostasis and that the motility defects in a digR mutant are instigated by the abnormal cell envelope and abnormal expression of agmQ and cheY4.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.