ArticleJournal of bacteriology2006
Mutations and rearrangements in the genome of Sulfolobus solfataricus P2.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 61 citations in OpenAlex.
- Probing the habitability of potential sulfuric acid rich subsurface lakes in Europa's ice shell viaFrontiers in microbiology · 2026Article
- The biology of thermoacidophilic archaea from the order Sulfolobales.FEMS microbiology reviews · 2021Article
- Archaeal tyrosine recombinases.FEMS microbiology reviews · 2021Review
- Interplay of Various Evolutionary Modes in Genome Diversification and Adaptive Evolution of the FamilyFrontiers in microbiology · 2021Article
- Article
- Functional Analysis of the NucS/EndoMS of the Hyperthermophilic ArchaeonFrontiers in microbiology · 2020Article
- Acid Experimental Evolution of the HaloarchaeonFrontiers in microbiology · 2020Article
- Nonmutational mechanism of inheritance in the ArchaeonProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Copy number variation is associated with gene expression change in archaea.Microbial genomics · 2018Article
- Evolution of Genome Architecture in Archaea: Spontaneous Generation of a New Chromosome in Haloferax volcanii.Molecular biology and evolution · 2018Article
- How a Genetically Stable Extremophile Evolves: Modes of Genome Diversification in the Archaeon Sulfolobus acidocaldarius.Journal of bacteriology · 2017Article
- Mechanisms of gene flow in archaea.Nature reviews. Microbiology · 2017Review
- Diverse CRISPR-Cas responses and dramatic cellular DNA changes and cell death in pKEF9-conjugated Sulfolobus species.Nucleic acids research · 2016Article
- The apt/6-Methylpurine Counterselection System and Its Applications in Genetic Studies of the Hyperthermophilic Archaeon Sulfolobus islandicus.Applied and environmental microbiology · 2016Article
- Expanding the Limits of Thermoacidophily in the Archaeon Sulfolobus solfataricus by Adaptive Evolution.Applied and environmental microbiology · 2016Article
- CRISPR-Cas Adaptive Immune Systems of the Sulfolobales: Unravelling Their Complexity and Diversity.Life (Basel, Switzerland) · 2015Review
- Unveiling cell surface and type IV secretion proteins responsible for archaeal rudivirus entry.Journal of virology · 2014Article
- Antisense regulation by transposon-derived RNAs in the hyperthermophilic archaeon Sulfolobus solfataricus.EMBO reports · 2013Article
- Novel insights into gene regulation of the rudivirus SIRV2 infecting Sulfolobus cells.RNA biology · 2013Article
- Genomics and genetics of Sulfolobus islandicus LAL14/1, a model hyperthermophilic archaeon.Open biology · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genome of Sulfolobus solfataricus P2 carries a larger number of transposable elements than any other sequenced genome from an archaeon or bacterium and, as a consequence, may be particularly susceptible to rearrangement and change. In order to gain more insight into the natures and frequencies of different types of mutation and possible rearrangements that can occur in the genome, the pyrEF locus was examined for mutations that were isolated after selection with 5-fluoroorotic acid. About two-thirds of the 130 mutations resulted from insertions of mobile elements, including insertion sequence (IS) elements and a single nonautonomous mobile element, SM2. For each of these, the element was identified and shown to be present at its original genomic position, consistent with a progressive increase in the copy numbers of the mobile elements. In addition, several base pair substitutions, as well as small deletions, insertions, and a duplication, were observed, and about one-fifth of the mutations occurred elsewhere in the genome, possibly in an orotate transporter gene. One mutant exhibited a 5-kb genomic rearrangement at the pyrEF locus involving a two-step IS element-dependent reaction, and its boundaries were defined using a specially developed "in vitro library" strategy. Moreover, while searching for the donor mobile elements, evidence was found for two major changes that had occurred in the genome of strain P2, one constituting a single deletion of about 4% of the total genome (124 kb), while the other involved the inversion of a 25-kb region. Both were bordered by IS elements and were inferred to have arisen through recombination events. The results underline the caution required in working experimentally with an organism such as S. solfataricus with a continually changing genome.
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