ArticlePlanta2006
Nuclear fragmentation and DNA degradation during programmed cell death in petals of morning glory (Ipomoea nil).
Article in Planta, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 73 citations in OpenAlex.
- Petal senescence in cut roses.Horticulture research · 2026Article
- The Senescence of Cut Daffodil Flowers Correlates with Programmed Cell Death Symptoms.International journal of molecular sciences · 2025Article
- A chemical approach to extend flower longevity of Japanese morning glory via inhibition of master senescence regulator EPHEMERAL1.Nature plants · 2024Article
- Signalling cascades choreographing petal cell death: implications for postharvest quality.Plant molecular biology · 2024Review
- Extracellular Compounds from Pathogenic BacteriumBiology · 2022Article
- Comparative Transcriptome Analysis of Flower Senescence ofCurrent genomics · 2022Article
- Nitric oxide and ROS mediate autophagy and regulate Alternaria alternata toxin-induced cell death in tobacco BY-2 cells.Scientific reports · 2019Article
- Relationship between loss of desiccation tolerance and programmed cell death (PCD) in mung bean (Vigna radiata) seeds.PloS one · 2019Article
- Autophagy counteracts instantaneous cell death during seasonal senescence of the fine roots and leaves in Populus trichocarpa.BMC plant biology · 2018Article
- Molecular aspects of flower senescence and strategies to improve flower longevity.Breeding science · 2018Review
- Length of the dark period affects flower opening and the expression of circadian-clock associated genes as well as xyloglucan endotransglucosylase/hydrolase genes in petals of morning glory (Ipomoea nil).Plant cell reports · 2014Article
- Mineral nutrient remobilization during corolla senescence in ethylene-sensitive and -insensitive flowers.AoB PLANTS · 2013Article
- Never say dye: new roles for an old fluorochrome.Plant signaling & behavior · 2012Review
- In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning.Journal of experimental botany · 2012Article
- Dealing with the problem of non-specific in situ mRNA hybridization signals associated with plant tissues undergoing programmed cell death.Plant methods · 2010Article
- InPSR26, a putative membrane protein, regulates programmed cell death during petal senescence in Japanese morning glory.Plant physiology · 2009Article
- Integrated signaling in flower senescence: an overview.Plant signaling & behavior · 2007Article
- Relationship between petal abscission and programmed cell death in Prunus yedoensis and Delphinium belladonna.Planta · 2007Article
- Gene expression in opening and senescing petals of morning glory (Ipomoea nil) flowers.Plant cell reports · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We studied DNA degradation and nuclear fragmentation during programmed cell death (PCD) in petals of Ipomoea nil (L.) Roth flowers. The DNA degradation, as observed on agarose gels, showed a large increase. Using DAPI, which stains DNA, and flow cytometry for DAPI fluorescence, we found that the number of DNA masses per petal at least doubled. This indicated chromatin fragmentation, either inside or outside the nucleus. Staining with the cationic lipophilic fluoroprobe DiOC6 indicated that each DNA mass had an external membrane. Fluorescence microscopy of the nuclei and DNA masses revealed an initial decrease in diameter together with chromatin condensation. The diameters of these condensed nuclei were about 70% of original. Two populations of nuclear diameter, one with an average diameter about half of the other, were observed at initial stages of nuclear fragmentation. The diameter of the DNA masses then gradually decreased further. The smallest observed DNA masses had a diameter less than 10% of that of the original nucleus. Cycloheximide treatment arrested the cytometrically determined changes in DNA fluorescence, indicating protein synthesis requirement. Ethylene inhibitors (AVG and 1-MCP) had no effect on the cytometrically determined DNA changes, suggesting that these processes are not controlled by endogenous ethylene.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.