ArticleGene therapy2006
Using magnetic forces to enhance non-viral gene transfer to airway epithelium in vivo.
Article in Gene therapy, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 89 citations in OpenAlex.
- Improved in-vivo airway gene transfer via magnetic-guidance, with protocol development informed by synchrotron imaging.Scientific reports · 2022Article
- Nonviral Locally Injected Magnetic Vectors for In Vivo Gene Delivery: A Review of Studies on Magnetofection.Nanomaterials (Basel, Switzerland) · 2021Review
- In vivo magnetofection: a novel approach for targeted topical delivery of nucleic acids for rectoanal motility disorders.American journal of physiology. Gastrointestinal and liver physiology · 2018Article
- Article
- Aerosol Delivery of siRNA to the Lungs. Part 1: Rationale for Gene Delivery Systems.Kona : powder science and technology in Japan · 2016Article
- Iron oxide nanoparticles for magnetically-guided and magnetically-responsive drug delivery.International journal of molecular sciences · 2015Review
- Functional magnetic nanoparticles for non-viral gene delivery and MR imaging.Pharmaceutical research · 2014Review
- Magnetofection: a reproducible method for gene delivery to melanoma cells.BioMed research international · 2013Article
- Polyethylenimine functionalized magnetic nanoparticles as a potential non-viral vector for gene delivery.Journal of materials science. Materials in medicine · 2012Article
- Phoenix rising: gene therapy makes a comeback.Acta biochimica et biophysica Sinica · 2012Review
- Pulmonary delivery of therapeutic siRNA.Advanced drug delivery reviews · 2012Review
- Magnetically enhanced nucleic acid delivery. Ten years of magnetofection-progress and prospects.Advanced drug delivery reviews · 2011Review
- Application of magnetic nanoparticles in pharmaceutical sciences.Pharmaceutical research · 2011Review
- Magnetic nanoparticles as targeted delivery systems in oncology.Radiology and oncology · 2011Article
- Gene transfer: how can the biological barriers be overcome?The Journal of membrane biology · 2010Article
- Iron oxide-based nanomagnets in nanomedicine: fabrication and applications.Nano reviews · 2010Article
- Physical methods of nucleic acid transfer: general concepts and applications.British journal of pharmacology · 2009Review
- Extracellular barriers in respiratory gene therapy.Advanced drug delivery reviews · 2009Review
- Cationic lipid-coated magnetic nanoparticles associated with transferrin for gene delivery.International journal of pharmaceutics · 2008Article
- Magnetically driven plasmid DNA delivery with biodegradable polymeric nanoparticles.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have assessed whether magnetic forces (magnetofection) can enhance non-viral gene transfer to the airways. TransMAG(PEI), a superparamagnetic particle was coupled to Lipofectamine 2000 or cationic lipid 67 (GL67)/plasmid DNA (pDNA) liposome complexes. In vitro transfection with these formulations resulted in approximately 300- and 30-fold increase in reporter gene expression, respectively, after exposure to a magnetic field, but only at suboptimal pDNA concentrations. Because GL67 has been formulated for in vivo use, we next assessed TransMAG(PEI) in the murine nasal epithelium in vivo, and compared this to naked pDNA. At the concentrations required for in vivo experiments, precipitation of magnetic complexes was seen. After extensive optimization, addition of non-precipitated magnetic particles resulted in approximately seven- and 90-fold decrease in gene expression for naked pDNA and GL67/pDNA liposome complexes, respectively, compared to non-magnetic particles. Thus, whereas exposure to a magnetic field improved in vitro transfection efficiency, translation to the in vivo setting remains difficult.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.